Bedossa P, Lemaigre G, Bacci J, Martin E
Laboratoire d'Anatomie Pathologique, Hôpital Antoine-Béclère, Clamart, France.
Digestion. 1989;44(1):7-13. doi: 10.1159/000199886.
The aims of the study were to measure the collagen content in pancreas using a colorimetric method and to compare the amount of collagen in normal pancreas (11 cases), diffuse fibrosing pancreatitis (17 cases), and chronic calcifying pancreatitis (11 cases). The procedure of fibrosis measurement was based on the selective capacity of two dyes, Sirius red and fast green, to set on collagen and noncollagenous proteins, respectively. After staining of sections, colors were eluted from the sections and the specific absorbance of each eluted dye was read on a spectrophotometer. The collagen content in normal pancreata was 26.5 +/- 7.2 micrograms collagen/mg protein. The amount of collagen increased with the age of patients: the mean value of the patients under the age of 50 was 18.2 +/- 4 micrograms collagen/mg protein whereas the mean value in older patients was 31.9 +/- 8 micrograms collagen/mg protein (p less than 0.01). The value of collagen in pancreas with a diffuse fibrosing pancreatitis was 44.7 +/- 7.5 micrograms collagen/mg protein. This value was significantly different from the collagen content in normal pancreas (p less than 0.001) and in pancreas with a chronic calcifying pancreatitis (77.9 +/- 8 micrograms collagen/mg protein, p less than 0.001). This method permits discrimination between different chronic diseases that can also be differentiated on a histopathologic basis.
本研究的目的是采用比色法测量胰腺中的胶原蛋白含量，并比较正常胰腺（11例）、弥漫性纤维化胰腺炎（17例）和慢性钙化性胰腺炎（11例）中的胶原蛋白含量。纤维化测量程序基于两种染料，天狼星红和固绿，分别对胶原蛋白和非胶原蛋白的选择性结合能力。切片染色后，从切片中洗脱颜色，并在分光光度计上读取每种洗脱染料的特定吸光度。正常胰腺中的胶原蛋白含量为26.5±7.2微克胶原蛋白/毫克蛋白质。胶原蛋白含量随患者年龄增加而增加：50岁以下患者的平均值为18.2±4微克胶原蛋白/毫克蛋白质，而老年患者的平均值为31.9±8微克胶原蛋白/毫克蛋白质（p<0.01）。弥漫性纤维化胰腺炎胰腺中的胶原蛋白值为44.7±7.5微克胶原蛋白/毫克蛋白质。该值与正常胰腺中的胶原蛋白含量（p<0.001）以及慢性钙化性胰腺炎胰腺中的胶原蛋白含量（77.9±8微克胶原蛋白/毫克蛋白质，p<0.001）有显著差异。该方法能够区分不同的慢性疾病，这些疾病也可在组织病理学基础上进行区分。
