Balm P H, Lambert J D, Wendelaar Bonga S E
Department of Animal Physiology, University of Nijmegen, The Netherlands.
Gen Comp Endocrinol. 1989 Oct;76(1):53-62. doi: 10.1016/0016-6480(89)90032-4.
Applying high-performance liquid chromatography and thin-layer chromatography to separate corticosteroids, we studied the biosynthesis of steroids by the interrenal cells of the head kidneys (the adrenocortical homolog) of Oreochromis mossambicus. Intact head kidneys converted exogenous 17 alpha-hydroxyprogesterone into mainly cortisol, but 11-deoxycortisol, cortisone, and androstenedione were also recovered from the medium. Incubation of intact tissue with pregnenolone in addition resulted in the formation of large amounts of an unidentified product, which was absent in incubations of tissue homogenates with pregnenolone.
应用高效液相色谱法和薄层色谱法分离皮质类固醇，我们研究了莫桑比克罗非鱼头部肾脏（肾上腺皮质同源物）的肾上腺皮质细胞中类固醇的生物合成。完整的头部肾脏将外源性17α-羟孕酮主要转化为皮质醇，但培养基中也检测到了11-脱氧皮质醇、可的松和雄烯二酮。完整组织与孕烯醇酮一起孵育还会产生大量未知产物，而组织匀浆与孕烯醇酮孵育时则没有这种产物。
