通过翻译增强子提高猪圆环病毒2型（PCV2）病毒样颗粒在Sf9细胞中的产量。
Enhanced production of porcine circovirus type 2 (PCV2) virus-like particles in Sf9 cells by translational enhancers.
作者信息
Liu Yangkun, Zhang Yuanyuan, Yao Lunguang, Hao Huafang, Fu Xiangjing, Yang Zengqi, Du Enqi
机构信息
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China,
出版信息
Biotechnol Lett. 2015 Sep;37(9):1765-71. doi: 10.1007/s10529-015-1856-7. Epub 2015 May 21.
OBJECTIVE
To investigate the effect of three translational enhancers for enhancing transgene expression in baculovirus expression vector system using GFP as a reporter gene and selected translational enhancers to increase porcine circovirus type 2 (PCV2) VLPs production.
RESULTS
P10UTR (the 3'-untranslated region from the baculovirus p10 gene), Syn21 (a synthetic AT-rich 21-bp sequence) and P10UTR/Syn21 increased the GFP yield by 1.4-, 4- and 4.8-fold, respectively. While IVS (intron from Drosophila myosin heavy chain gene) decreased the GFP yield by 65%. Moreover, the synergy of P10UTR/Syn21 increased the yield of PCV2 VLPs by 4.1 fold (45 μg/10(6) cells) compared with standard baculovirus vector.
CONCLUSION
The synergy of P10UTR/Syn21 is a potential strategy to improve the recombinant vaccine production besides PCV2 VLPs in BEVS.
目的
以绿色荧光蛋白（GFP）作为报告基因，研究三种翻译增强子在杆状病毒表达载体系统中增强转基因表达的效果，并选择翻译增强子以提高猪圆环病毒2型（PCV2）病毒样颗粒（VLPs）的产量。
结果
P10UTR（杆状病毒p10基因的3'非翻译区）、Syn21（一段富含AT的21bp合成序列）和P10UTR/Syn21分别使GFP产量提高了1.4倍、4倍和4.8倍。而内含子（果蝇肌球蛋白重链基因的内含子）使GFP产量降低了65%。此外，与标准杆状病毒载体相比，P10UTR/Syn21的协同作用使PCV2 VLPs的产量提高了4.1倍（45μg/10⁶个细胞）。
结论
P10UTR/Syn21的协同作用是除了在杆状病毒表达系统中提高PCV2 VLPs产量外，提高重组疫苗产量的一种潜在策略。
Zotero 插件