Nakamura Takahiro, Hata Yuiko, Nagata Maho, Yokoi Norihiko, Yamaguchi Shumpei, Kaku Taiichi, Kinoshita Shigeru
1] Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan [2] Research Center for Inflammation and Regenerative Medicine, Doshisha University, Kyoto, Japan.
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Sci Rep. 2015 May 21;5:10248. doi: 10.1038/srep10248.
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.
干眼综合征（DES）是一种累及泪液和眼表的多因素疾病，是最常见的眼部疾病之一。泪膜含有眼黏蛋白，对维持湿润眼表的稳态至关重要。由于治疗DES的临床选择有限，因此需要更多新型治疗方法来改善临床疗效。在本研究中，我们发现胎盘提取物衍生的二肽（JBP485）能显著促进兔结膜上皮中凝胶形成黏蛋白5ac（Muc5ac）的表达和分泌。JBP485还提高了兔角膜上皮中细胞表面相关黏蛋白（Muc1/4/16）的表达水平。泪液分泌试验结果表明，JBP485可诱导兔模型泪液分泌。此外，JBP485在小鼠干眼模型中可在临床上改善角膜上皮损伤。因此，我们的数据表明，JBP485能有效促进兔眼表上皮中黏蛋白和水性泪液的分泌，有潜力作为DES的一种新型治疗方法。
