膜重塑机器的结构与功能研究
Structural and functional studies of membrane remodeling machines.
作者信息
Kalia Raghav, Talledge Nathaniel, Frost Adam
机构信息
Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, UT, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
出版信息
Methods Cell Biol. 2015;128:165-200. doi: 10.1016/bs.mcb.2015.02.007. Epub 2015 Apr 8.
Abstract
Building cells from their component parts will hinge upon our ability to reconstitute biochemical compartmentalization and exchange between membrane-delimited organelles. By contrast with our understanding of other cellular events, the mechanisms that govern membrane trafficking has lagged because the presence of phospholipid bilayers complicates the use of standard methods. This chapter describes in vitro methods for purifying, reconstituting, and visualizing membrane remodeling activities directly by electron cryomicroscopy.
摘要
利用细胞的组成部分构建细胞将取决于我们重建生物化学区室化以及膜界定细胞器之间交换的能力。与我们对其他细胞事件的理解相比，由于磷脂双分子层的存在使标准方法的使用变得复杂，因此调控膜运输的机制一直滞后。本章介绍了通过电子冷冻显微镜直接纯化、重建和观察膜重塑活动的体外方法。
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