外泌体长链非编码RNA-p21的水平可能有助于区分前列腺癌和良性疾病。

Işın Mustafa, Uysaler Ege, Özgür Emre, Köseoğlu Hikmet, Şanlı Öner, Yücel Ömer B, Gezer Uğur, Dalay Nejat

Department of Basic Oncology, Oncology Institute, Istanbul University , Istanbul, Turkey.

Department of Urology, School of Medicine, Istanbul Hospital, Başkent University , Istanbul, Turkey.

Front Genet. 2015 May 6;6:168. doi: 10.3389/fgene.2015.00168. eCollection 2015.

Exosomes are membranous vesicles containing various biomolecules including lncRNAs which are involved in cellular communication and are secreted from many cells including cancer cells. In our study, investigated the exosomal GAS5 and lincRNA-p21 lncRNA levels in urine samples from 30 patients with prostate cancer (PCa) and 49 patients with benign prostatic hyperplasia. Quantification of lncRNA molecules was performed by real-time PCR. We observed a significant difference in the exosomal lincRNA-p21 levels between PCa and BPH patients whereas the GAS5 levels did not reveal a difference. Our data suggest that the discriminative potential of exosomal lincRNA-p21 levels may help to improve the diagnostic prediction of the malignant state for patients with PCa.

外泌体是包含多种生物分子的膜性囊泡，其中包括参与细胞通讯的长链非编码RNA（lncRNA），并且可从包括癌细胞在内的许多细胞中分泌。在我们的研究中，我们检测了30例前列腺癌（PCa）患者和49例良性前列腺增生患者尿液样本中的外泌体GAS5和lincRNA-p21 lncRNA水平。通过实时PCR对lncRNA分子进行定量分析。我们观察到PCa患者和BPH患者之间外泌体lincRNA-p21水平存在显著差异，而GAS5水平没有差异。我们的数据表明，外泌体lincRNA-p21水平的鉴别潜力可能有助于改善PCa患者恶性状态的诊断预测。