马来布鲁线虫中微小RNA活性的功能分析
Functional analysis of microRNA activity in Brugia malayi.
作者信息
Liu Canhui, Voronin Denis, Poole Catherine B, Bachu Saheed, Rogers Matthew B, Jin Jingmin, Ghedin Elodie, Lustigman Sara, McReynolds Larry A, Unnasch Thomas R
机构信息
Global Health Infectious Disease Research Program, Department of Global Health, College of Public Health, University of South Florida, 3720 Spectrum Blvd, Suite 304, Tampa, FL 33612, USA.
New York Blood Center, Lindsley F. Kimball Research Institute, 310 East 67th Street, New York, NY 10065, USA.
出版信息
Int J Parasitol. 2015 Aug;45(9-10):579-83. doi: 10.1016/j.ijpara.2015.04.004. Epub 2015 May 22.
The complement of the Brugia malayi microRNA-71 was inserted into the 3' untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3' untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets.
马来布鲁线虫微小RNA - 71的互补序列被插入到报告质粒的3'非翻译区，导致报告基因活性降低。种子序列的突变恢复了活性。将两个算法预测的假定靶基因的3'非翻译区插入报告基因中，导致活性出现类似降低；预测靶序列的突变恢复了活性。这些实验表明，可以使用当前算法预测马来布鲁线虫微小RNA的靶标，并描述了一种功能测定法来确认预测的靶标。
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