Dumas S, Darmon M C, Delort J, Mallet J
Laboratoire de Neurobilogie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
J Neurosci Res. 1989 Dec;24(4):537-47. doi: 10.1002/jnr.490240412.
In a previous study, we characterized two tryptophan hydroxylase mRNAs (TPH mRNAs) in the pineal gland. However, we failed to detect these species in the raphe by Northern blot experiments. Here, we report by S1 nuclease analysis and in situ hybridization that these two TPH mRNAs, as well as a third species, are expressed both in pineal gland and in raphe. In both tissues, the three mRNAs are transcribed predominantly from the same promoter. Strikingly, from the results of S1 maping analysis, it was observed that the total level of TPH mRNA per tissue is at least 150 times lower in the raphe than in the pineal gland. In contrast, TPH antigen as quantified by immunoblot experiments is about threefold more abundant per raphe than per pineal gland. TPH mRNA from one raphe and one pineal gland yield in vitro about the same amount of TPH antigen, suggesting that the discrepancy in the ratios of TPH mRNA and TPH antigen between the raphe and the pineal gland results, at least in part, from a difference in the translation efficiency of TPH mRNAs in the two structures.
在先前的一项研究中，我们鉴定了松果体中的两种色氨酸羟化酶mRNA（TPH mRNA）。然而，我们通过Northern印迹实验未能在中缝核中检测到这些种类。在此，我们通过S1核酸酶分析和原位杂交报告，这两种TPH mRNA以及第三种mRNA在松果体和中缝核中均有表达。在这两种组织中，这三种mRNA主要从同一个启动子转录而来。引人注目的是，从S1图谱分析的结果来看，观察到每组织中TPH mRNA的总水平在中缝核中比在松果体中至少低150倍。相比之下，通过免疫印迹实验定量的TPH抗原在每中缝核中的丰度比每松果体中的约高三倍。来自一个中缝核和一个松果体的TPH mRNA在体外产生的TPH抗原量大致相同，这表明中缝核和松果体之间TPH mRNA与TPH抗原比例的差异至少部分是由于这两种结构中TPH mRNA翻译效率的差异所致。
