Sharma Dhara, Rawat Indu, Goel H C
Indian J Exp Biol. 2015 Apr;53(4):216-21.
In this study, we investigated few dietary cucurbits for anticancer activity by monitoring cytotoxic (MTT and LDH assays), apoptotic (caspase-3 and annexin-V assays), and also their anti-inflammatory effects by IL-8 cytokine assay. Aqua-alcoholic (50:50) whole extracts of cucurbits [Lagenaria siceraria (Ls), Luffa cylindrica (Lc) and Cucurbita pepo (Cp)] were evaluated in colon cancer cells (HT-29 and HCT-15) and were compared with isolated biomolecule, cucurbitacin-B (Cbit-B). MTT and LDH assays revealed that the cucurbit extracts and Cbit-B, in a concentration dependent manner, decreased the viability of HT-29 and HCT-15 cells substantially. The viability of lymphocytes was, however, only marginally decreased, yielding a potential advantage over the tumor cells. Caspase-3 assay revealed maximum apoptosis with Ls while annexin V assay demonstrated maximum efficacy of Lc in this context. These cucurbits have also shown decreased secretion of IL-8, thereby revealing their anti-inflammatory capability. The results have demonstrated the therapeutic potential of dietary cucurbits in inhibiting cancer and inflammatory cytokine.
在本研究中，我们通过监测细胞毒性（MTT和LDH检测）、凋亡（caspase-3和膜联蛋白V检测）来研究几种食用葫芦科植物的抗癌活性，并通过IL-8细胞因子检测来研究它们的抗炎作用。对葫芦科植物[葫芦（Ls）、丝瓜（Lc）和西葫芦（Cp）]的水醇（50:50）全提取物在结肠癌细胞（HT-29和HCT-15）中进行了评估，并与分离出的生物分子葫芦素-B（Cbit-B）进行了比较。MTT和LDH检测显示，葫芦提取物和Cbit-B以浓度依赖性方式显著降低了HT-29和HCT-15细胞的活力。然而，淋巴细胞的活力仅略有下降，相对于肿瘤细胞具有潜在优势。Caspase-3检测显示葫芦的凋亡作用最强，而在这种情况下膜联蛋白V检测表明丝瓜的效果最佳。这些葫芦科植物还显示出IL-8分泌减少，从而揭示了它们的抗炎能力。结果证明了食用葫芦科植物在抑制癌症和炎性细胞因子方面的治疗潜力。
