Li Xiaowei, Xu Hongxing, Feng Ling, Fu Xiao, Zhang Yalin, Nansen Christian
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, Northwest A&F University, Yangling, Shaanxi, China.
PLoS One. 2017 May 4;12(5):e0176392. doi: 10.1371/journal.pone.0176392. eCollection 2017.
Proximal imaging remote sensing technologies are used to phenotype and to characterize organisms based on specific external body reflectance features. These imaging technologies are gaining interest and becoming more widely used and applied in ecological, systematic, evolutionary, and physiological studies of plants and also of animals. However, important factors may impact the quality and consistency of body reflectance features and therefore the ability to use these technologies as part of non-invasive phenotyping and characterization of organisms. We acquired hyperspectral body reflectance profiles from three insect species, and we examined how preparation procedures and preservation time affected the ability to detect reflectance responses to gender, origin, and age. Different portions of the radiometric spectrum varied markedly in their sensitivity to preparation procedures and preservation time. Based on studies of three insect species, we successfully identified specific radiometric regions, in which phenotypic traits become significantly more pronounced based on either: 1) gentle cleaning of museum specimens with distilled water, or 2) killing and preserving insect specimens in 70% ethanol. Standardization of killing and preservation procedures will greatly increase the ability to use proximal imaging remote sensing technologies as part of phenotyping and also when used in ecological and evolutionary studies of invertebrates.
近端成像遥感技术用于根据生物体特定的体表反射特征对其进行表型分析和特征描述。这些成像技术正受到越来越多的关注，并在植物和动物的生态、系统、进化及生理学研究中得到更广泛的应用。然而，一些重要因素可能会影响体表反射特征的质量和一致性，进而影响将这些技术用于生物体非侵入性表型分析和特征描述的能力。我们获取了三种昆虫的高光谱体表反射图谱，并研究了制备程序和保存时间如何影响检测对性别、来源和年龄的反射响应的能力。辐射光谱的不同部分对制备程序和保存时间的敏感性差异显著。基于对三种昆虫的研究，我们成功识别出了特定的辐射区域，在这些区域中，基于以下两种情况之一，表型特征会变得更加明显：1）用蒸馏水轻柔清洗博物馆标本；2）将昆虫标本置于70%乙醇中杀死并保存。杀死和保存程序的标准化将大大提高近端成像遥感技术作为表型分析一部分的使用能力，以及在无脊椎动物生态和进化研究中的应用能力。
