Yezdani Arif, Mahalakshmi Krishnan, Padmavathy Kesavaram
Department of Orthodontics and Dentofacial Orthopedics Sree Balaji Dental College and Hospital, Bharath University, Chennai, Tamil Nadu, India.
Department of Microbiology, Sree Balaji Dental College and Hospital, Bharath University, Chennai, Tamil Nadu, India.
J Pharm Bioallied Sci. 2015 Apr;7(Suppl 1):S111-5. doi: 10.4103/0975-7406.155847.
This study was designed to evaluate the efficiency of microwave sterilization of orthodontic instruments and molar bands immersed in plain distilled water with and without oral rinse, and to ascertain the minimum time of exposure required to sterilize.
The orthodontic instruments (hinged and nonhinged), molar bands and mouth mirrorsused in the patient 's mouth were selected for the study. The instruments were divided into two groups - Group I with oral rinse-set A (0.01% chlorhexidine gluconate) and set B (0.025% betadine) and Group II (included sets C and D without oral rinse). The instruments of set A, B and C were microwaved at 2,450 MHz, 800 W for 5 min, whereas, set D was microwaved for 10 min at the same above mentioned specifications. The efficacy of sterilization was assessed by stab inoculation of the instruments onto trypticase soya agar plates. The plates were checked for bacterial growth following incubation at 37 °C for 24 h. For sterility control,Geobacillus stearothermophilus (MTCC 1518) was included.
No growth was observed in the plates that were inoculated with the microwaved orthodontic instruments of sets A, B and D, whereas scanty bacterial growth was observed in the plates inoculatedwith the microwaved set C instruments.
Effective sterilization was achieved when the orthodontic instruments and molar bands were immersed in distilled water without oral rinse and microwaved for 10 min as also for those that were immersed in distilled water with oral rinse and microwaved for 5 min.
本研究旨在评估正畸器械和磨牙带在有无口腔冲洗液的情况下浸泡于纯蒸馏水中进行微波灭菌的效率，并确定灭菌所需的最短暴露时间。
选取患者口腔中使用的正畸器械（有铰链和无铰链的）、磨牙带和口镜用于本研究。器械分为两组——第一组有口腔冲洗液，A组（0.01%葡萄糖酸氯己定）和B组（0.025%聚维酮碘）；第二组（包括C组和D组，无口腔冲洗液）。A组、B组和C组的器械在2450MHz、800W下微波处理5分钟，而D组器械在上述相同规格下微波处理10分钟。通过将器械穿刺接种到胰蛋白酶大豆琼脂平板上来评估灭菌效果。将平板在37°C孵育24小时后检查细菌生长情况。为进行无菌对照，纳入嗜热栖热放线菌（MTCC 1518）。
接种了A组、B组和D组微波处理后的正畸器械的平板上未观察到生长，而接种了C组微波处理后的器械的平板上观察到少量细菌生长。
当正畸器械和磨牙带浸泡在无口腔冲洗液的蒸馏水中并微波处理10分钟时以及浸泡在有口腔冲洗液的蒸馏水中并微波处理5分钟时，均可实现有效灭菌。
