Webb B C, Thomas C J, Harty D W, Willcox M D
Institute of Dental Research, School of Dental Studies, Faculty of Dentistry, University of Sydney, NSW, Australia.
J Oral Rehabil. 1998 Jun;25(6):416-23. doi: 10.1046/j.1365-2842.1998.00266.x.
Species of Candida and in particular Candida albicans may be involved in the aetiology of denture stomatitis. Studies have shown that Candida and other oral micro-organisms including Streptococcus gordonii are associated with denture plaque; hence denture hygiene is an important factor in the prevention and treatment of the disease. The aim of this investigation was to test in vitro the efficacy of two methods of denture sterilization: (1) microwave irradiation and (2) sodium hypochlorite soak. Twenty upper acrylic dentures were prepared for microbiological assay; 10 were inoculated with C. albicans H1 and 10 with S. gordonii LGR2. Within each group, five dentures were tested in a domestic microwave oven for optimal exposure time and temperature to ensure sterilization; the five control dentures were not microwaved. Microbiological analyses showed that the inoculated dentures became sterile after six min of irradiation at medium setting (2450 MHz, 350 W). Damage to the microorganisms after microwave irradiation was clearly visible by scanning electron microscopy (SEM). Following the same protocol as above, experimental dentures were soaked for 8 h in either 0.02%, or 0.0125% sodium hypochlorite solution and control dentures soaked in distilled water. Microbiological analyses showed that the experimental dentures inoculated with C. albicans H1 became sterile. By contrast, those inoculated with S. gordonii LGR2 did not become sterile, and the SEM procedures confirmed these findings. The results of this study indicate that microwaving may be a more effective method of denture sterilization than denture soaking in sodium hypochlorite. However, compared with microwaving, hypochlorite reduces the levels of residual non-viable micro-organisms attached to the denture surface.
念珠菌属，尤其是白色念珠菌，可能与义齿性口炎的病因有关。研究表明，念珠菌及其他口腔微生物，包括戈登链球菌，都与义齿菌斑有关；因此，义齿卫生是预防和治疗该疾病的一个重要因素。本研究的目的是在体外测试两种义齿消毒方法的效果：（1）微波辐照和（2）次氯酸钠浸泡。制备了20副上颌丙烯酸义齿用于微生物检测；10副接种白色念珠菌H1，10副接种戈登链球菌LGR2。在每组中，5副义齿在家用微波炉中测试最佳暴露时间和温度以确保消毒；5副对照义齿未进行微波处理。微生物分析表明，接种后的义齿在中等功率设置（2450 MHz，350 W）下辐照6分钟后变得无菌。通过扫描电子显微镜（SEM）可以清楚地看到微波辐照后微生物的损伤。按照上述相同方案，将实验义齿浸泡在0.02%或0.0125%的次氯酸钠溶液中8小时，对照义齿浸泡在蒸馏水中。微生物分析表明，接种白色念珠菌H1的实验义齿变得无菌。相比之下，接种戈登链球菌LGR2的义齿未变得无菌，SEM程序证实了这些结果。本研究结果表明，微波消毒可能比义齿浸泡在次氯酸钠中更有效地消毒义齿。然而，与微波消毒相比，次氯酸钠可降低附着在义齿表面的残留非存活微生物水平。
