Zeng Cong-cong, Liu Xi, Liu Wang-wang, Wang Ling, Cheng Jin-guo, Chen San-mei, Chen Guo-rong
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2015 Jan;31(1):38-42.
To observe the effect and mechanism of curcumin derivative B06 on kidney from rats with hyperlipidemia and type 2 diabetes.
Thirty five male SD rats were randomly divided into five groups(n = 7): the normal control group, high-fat group, high-fat + B06-treatd group, diabetic group, diabetic + B06-treated group. After fed with high-fat diet for 4 weeks, the later two groups were in- jected with streptozotocin intraperitoneally to induce type 2 diabetes mellitus. B06-treated groups were given B06 by gavage at a dosage of 0.2 mg/kg . d for 8 weeks. After the treatment, the serum creatinine, blood urea nitrogen and uric acid were detected biochemically, the morphology of kidney was observed with light and transmission electron microscopy, the expression of collagen fibers was observed with Masson staining, the protein expression of collogen IV and fibronectin in kidney were determined by Immunohistochemistry.
It was showed that the levels of the serum creatinine and blood urea nitrogen elevated significantly in diabetic group. In high-fat and diabetic groups, increased glomerular mesangial matrix and collagen fiber and thicken glomerular basal membrane were observed under light microscopy, swelling and fusion of foot process were found under electron microscope; increased green matrix within glomeruli was observed under Masson staining. collogen IV and fibronectin protein expression were significantly enhanced in high-fat group and diabetic group. After B06's intervention, the levels of serum creatinine and blood urea nitrogen were decreased in diabetic groups, the morphological change of kidney was obviously relieved, Collogen IV and fibronectin protein expression reduced.
Curcumin derivative B06 exerts a protective effect on kidney in type 2 diabetic rats, reduced expressions of collogen IV and fibronectin, inhibition of the accumulation of extracellular matrix and glomerular mesangial proliferation, and then prevention of renal fibrosis may be the mechanism.
观察姜黄素衍生物B06对高脂血症合并2型糖尿病大鼠肾脏的影响及其机制。
将35只雄性SD大鼠随机分为5组（n = 7）：正常对照组、高脂组、高脂 + B06治疗组、糖尿病组、糖尿病 + B06治疗组。高脂饮食喂养4周后，后两组腹腔注射链脲佐菌素诱导2型糖尿病。B06治疗组按0.2 mg/kg·d的剂量灌胃给予B06，共8周。治疗后，生化检测血清肌酐、血尿素氮和尿酸，光镜和透射电镜观察肾脏形态，Masson染色观察胶原纤维表达，免疫组化法检测肾脏中IV型胶原和纤连蛋白的蛋白表达。
结果显示糖尿病组血清肌酐和血尿素氮水平显著升高。光镜下，高脂组和糖尿病组肾小球系膜基质和胶原纤维增多，肾小球基底膜增厚；电镜下可见足突肿胀、融合；Masson染色显示肾小球内绿色基质增多。高脂组和糖尿病组IV型胶原和纤连蛋白蛋白表达显著增强。B06干预后，糖尿病组血清肌酐和血尿素氮水平降低，肾脏形态学改变明显减轻，IV型胶原和纤连蛋白蛋白表达减少。
姜黄素衍生物B06对2型糖尿病大鼠肾脏具有保护作用，降低IV型胶原和纤连蛋白的表达，抑制细胞外基质积聚和肾小球系膜增生，进而预防肾纤维化可能是其机制。
