[儿童戊型肝炎标志物的患病率]

[Prevalence of hepatitis E markers in children].

作者信息

Potemkin I A, Lopatukhina M A, Gadzhieva O A, Prokhorova E L, Diyarrassuba A, Isaeva O A, Kozhanova T V, Ivanova O E, Silenova O V, Setdikova N Kh, Kyuregyan K K, Mikhailov M I

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2015 Mar-Apr(2):38-46.

PMID:26016342

Abstract

AIM

Frequency of detection determination for past and current hepatitis E virus (HEV) infection markers in children with immune suppression, as well as children with normal immune status.

MATERIALS AND METHODS

The presence of HEV markers (anti-HEV IgG and IgM, HEV RNA) was studied in 609 sera samples of children with neurologic pathologies, 87 samples--from children with immune deficiencies, as well as 3122 samples from conditionally healthy children of 6 regions of Russia. The children were divided into 5 age groups. Anti-HEV IgG and IgM determination was carried out in EIA, HEV RNA--by RT-PCR.

RESULTS

The frequency of detection of anamnestic anti-HEV IgG turned out to be significantly higher among immune-compromised. children compared with healthy children (5.7% against 1.4%, p < 0.05). Anti-HEV IgM, that testify to current or recent infection, were also detected significantly more frequently among children with immune-suppression (1.1-1.6%) compared with healthy children (0.25%, p < 0.05). HEV RNA was detected in 1 child with the absence of anti-HEV IgM and IgG. Nucleotide sequence analysis of HEV confirmed membership of this isolate in genotype 3, that is prevalent in non-endemic territories.

CONCLUSION

The data obtained have demonstrated, that HEV-infection is prevalent among children in Russia and its course is, probably, asymptomatic in most cases. Immune suppression is a factor of increased risk of infection of children with HEV.

摘要

目的

确定免疫抑制儿童以及免疫状态正常儿童中既往和当前戊型肝炎病毒（HEV）感染标志物的检测频率。

材料与方法

对609例患有神经疾病儿童的血清样本、87例免疫缺陷儿童的血清样本以及来自俄罗斯6个地区的3122例健康儿童的血清样本进行HEV标志物（抗HEV IgG和IgM、HEV RNA）检测。将儿童分为5个年龄组。采用酶免疫分析（EIA）检测抗HEV IgG和IgM，采用逆转录聚合酶链反应（RT-PCR）检测HEV RNA。

结果

免疫功能低下儿童中既往抗HEV IgG的检测频率明显高于健康儿童（5.7% 对1.4%，p < 0.05）。与健康儿童（0.25%，p < 0.05）相比，免疫抑制儿童中抗HEV IgM（提示当前或近期感染）的检测频率也明显更高（1.1 - 1.6%）。在1例无抗HEV IgM和IgG的儿童中检测到HEV RNA。对HEV的核苷酸序列分析证实该分离株属于3型，此型在非流行地区较为常见。