Shin Min Jae, Kim Ye Jin, Kim Jong-Duk
Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Korea.
Soft Matter. 2015 Jul 7;11(25):5037-43. doi: 10.1039/c5sm00925a.
The noble vesicular system of polydiacetylene showed a red shift using two types of detecting systems. One of the systems involves the absorption of target materials from the outer side of the vesicle, and the other system involves the permeation through the vesicular layers from within the vesicle. The chromatic mixed vesicles of N-(2-aminoethyl)pentacosa-10,12-diynamide (AEPCDA) and dimethyldioctadecylammonium chloride (DODAC) were fabricated by sonication, followed by polymerization by UV irradiation. The stability of monomeric vesicles was observed to increase with the polymerization of the vesicles. Methotrexate was used as a target material. The polymerized mixed vesicles having a blue color were exposed to a concentration gradient of methotrexate, and a red shift was observed indicating the adsorption of methotrexate on the polydiacetylene bilayer. In order to check the chromatic change by the permeation of methotrexate, we separated the vesicle portion, which contained methotrexate inside the vesicle, and checked chromatic change during the permeation of methotrexate through the vesicle. The red shift apparently indicates the disturbance in the bilayer induced by the permeation of methotrexate. The maximum contrast of color appeared at the equal molar ratio of AEPCDA and DODAC, indicating that the formation of flexible and deformable vesicular layers is important for red shift. Therefore, it is hypothesized that the system can be applicable for the chromatic detection of the permeation of methotrexate through the polydiacetylene layer.
聚二乙炔的贵金属泡囊系统在使用两种检测系统时出现了红移。其中一种系统涉及从泡囊外侧吸收目标物质，另一种系统涉及从泡囊内部渗透过泡囊层。通过超声处理制备了N-(2-氨基乙基)二十五碳-10,12-二炔酰胺(AEPCDA)和二甲基二十八烷基氯化铵(DODAC)的彩色混合泡囊，随后通过紫外线照射进行聚合。观察到单体泡囊的稳定性随着泡囊的聚合而增加。甲氨蝶呤用作目标物质。将具有蓝色的聚合混合泡囊暴露于甲氨蝶呤的浓度梯度下，观察到红移，表明甲氨蝶呤吸附在聚二乙炔双分子层上。为了检查甲氨蝶呤渗透引起的颜色变化，我们分离了泡囊部分（泡囊内部含有甲氨蝶呤），并检查了甲氨蝶呤透过泡囊过程中的颜色变化。红移显然表明甲氨蝶呤的渗透引起了双分子层的扰动。在AEPCDA和DODAC等摩尔比时出现最大颜色对比度，表明形成柔性且可变形的泡囊层对红移很重要。因此，推测该系统可用于通过聚二乙炔层对甲氨蝶呤渗透进行颜色检测。
