Date I, Felten S Y, Felten D L
Department of Neurobiology and Anatomy, University of Rochester School of Medicine, NY 14642.
Neurosci Lett. 1989 Dec 4;106(3):282-6. doi: 10.1016/0304-3940(89)90177-8.
The administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to young (2-3 months) and aging (12 months) C57BL/6 mice (4 x 20 mg/kg, i.p., given 12 h apart) reduced tyrosine hydroxylase (TH)-immunoreactive (IR) fibers in the striatum, and reduced dopamine (DA) concentration to 28% of controls in young, and 16% of controls in aging mouse brain five weeks after administration. Although GM1 ganglioside treatment (30 mg/kg, i.p., daily for 5 weeks) restored striatal dopamine concentration to 74% of the control concentration in young mice, such an apparent recovery was not seen in aging brain. Immunocytochemical analysis also showed marked recovery of TH-IR fibers in the striatum of MPTP-depleted young mice treated with GM1 ganglioside while TH-IR fibers in the striatum of MPTP-depleted aging mice showed no recovery with such treatment. We conclude that treatment of MPTP-depleted young mice with GM1 ganglioside results in partial recovery in the striatal DA system, but such benefits do not extend to aging mice.
给年轻（2 - 3个月）和老龄（12个月）的C57BL/6小鼠腹腔注射1 - 甲基 - 4 - 苯基 - 1,2,3,6 - 四氢吡啶（MPTP）（4×20 mg/kg，间隔12小时给药），5周后，纹状体中酪氨酸羟化酶（TH）免疫反应性（IR）纤维减少，年轻小鼠脑内多巴胺（DA）浓度降至对照组的28%，老龄小鼠降至对照组的16%。尽管给予GM1神经节苷脂治疗（30 mg/kg，腹腔注射，每日1次，共5周）可使年轻小鼠纹状体多巴胺浓度恢复至对照组浓度的74%，但在老龄小鼠脑内未观察到这种明显的恢复。免疫细胞化学分析还显示，用GM1神经节苷脂治疗的MPTP处理的年轻小鼠纹状体中TH - IR纤维明显恢复，而MPTP处理的老龄小鼠纹状体中的TH - IR纤维经此治疗未恢复。我们得出结论，用GM1神经节苷脂治疗MPTP处理的年轻小鼠可使纹状体DA系统部分恢复，但这种益处不适用于老龄小鼠。
