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2
Phosphorylation of the leucocyte NADPH oxidase subunit p47(phox) by casein kinase 2: conformation-dependent phosphorylation and modulation of oxidase activity.酪蛋白激酶2对白细胞NADPH氧化酶亚基p47（phox）的磷酸化作用：构象依赖性磷酸化及氧化酶活性调节
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本文引用的文献

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Nox family NADPH oxidases: Molecular mechanisms of activation.Nox家族NADPH氧化酶：激活的分子机制
Free Radic Biol Med. 2014 Nov;76:208-26. doi: 10.1016/j.freeradbiomed.2014.07.046. Epub 2014 Aug 23.
2
Regulation by S-nitrosylation of protein post-translational modification.蛋白质翻译后修饰的 S-亚硝基化调节。
J Biol Chem. 2012 Feb 10;287(7):4411-8. doi: 10.1074/jbc.R111.285742. Epub 2011 Dec 6.
3
Nitric oxide suppresses NADPH oxidase-dependent superoxide production by S-nitrosylation in human endothelial cells.一氧化氮通过S-亚硝基化抑制人内皮细胞中NADPH氧化酶依赖性超氧化物的产生。
Cardiovasc Res. 2007 Jul 15;75(2):349-58. doi: 10.1016/j.cardiores.2007.03.030. Epub 2007 Apr 21.
4
Solution structure of the tandem Src homology 3 domains of p47phox in an autoinhibited form.处于自身抑制形式的p47phox串联Src同源3结构域的溶液结构。
J Biol Chem. 2004 Jul 9;279(28):29752-60. doi: 10.1074/jbc.M401457200. Epub 2004 Apr 29.
5
Inactivation of NADP+-dependent isocitrate dehydrogenase by peroxynitrite. Implications for cytotoxicity and alcohol-induced liver injury.过氧亚硝酸根使依赖烟酰胺腺嘌呤二核苷酸磷酸（NADP⁺）的异柠檬酸脱氢酶失活。对细胞毒性和酒精性肝损伤的影响。
J Biol Chem. 2003 Dec 19;278(51):51360-71. doi: 10.1074/jbc.M302332200. Epub 2003 Oct 9.
6
Phosphorylation of the leucocyte NADPH oxidase subunit p47(phox) by casein kinase 2: conformation-dependent phosphorylation and modulation of oxidase activity.酪蛋白激酶2对白细胞NADPH氧化酶亚基p47（phox）的磷酸化作用：构象依赖性磷酸化及氧化酶活性调节
Biochem J. 2001 Sep 15;358(Pt 3):783-90. doi: 10.1042/0264-6021:3580783.
7
C-terminal region of the cytosolic subunit p47(phox) is a primary target of conformational change during the activation of leukocyte NADPH oxidase.胞质亚基p47（phox）的C末端区域是白细胞NADPH氧化酶激活过程中构象变化的主要靶点。
Biochimie. 2000 Aug;82(8):727-32. doi: 10.1016/s0300-9084(00)01153-6.
8
Conformational changes of the leukocyte NADPH oxidase subunit p47(phox) during activation studied through its intrinsic fluorescence.通过其固有荧光研究白细胞NADPH氧化酶亚基p47（phox）在激活过程中的构象变化。
Biochim Biophys Acta. 1998 Sep 8;1387(1-2):406-14. doi: 10.1016/s0167-4838(98)00152-6.
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The leukocyte NADPH oxidase subunit p47PHOX: the role of the cysteine residues.
Arch Biochem Biophys. 1998 Feb 1;350(1):36-40. doi: 10.1006/abbi.1997.0484.
10
Activation of the leukocyte NADPH oxidase subunit p47phox by protein kinase C. A phosphorylation-dependent change in the conformation of the C-terminal end of p47phox.蛋白激酶C对白细胞NADPH氧化酶亚基p47phox的激活作用。p47phox C末端构象的磷酸化依赖性变化。
Biochemistry. 1997 Jun 17;36(24):7474-80. doi: 10.1021/bi9700936.

p47（phox）的S-亚硝基化增强了酪蛋白激酶2的磷酸化作用。

S-Nitrosylation of p47(phox) enhances phosphorylation by casein kinase 2.

作者信息

Kil In Sup, Lee Jin Hyup, Yoon Soo Hyun, Bae Young Seuk, Kim Seontae, Shin Seoung Woo, Park Jeen-Woo

出版信息

Redox Rep. 2015 Sep;20(5):228-33. doi: 10.1179/1351000215Y.0000000014. Epub 2015 May 27.

DOI:10.1179/1351000215Y.0000000014
PMID:26018922
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6837347/
Abstract

OBJECTIVES

Leukocyte NADPH oxidase, which is active in neutrophils, is a membrane-bound enzyme that catalyzes the reduction of oxygen to O2(-) by using NADPH as an electron donor. Previously, we reported that casein kinase 2 (CK2), a ubiquitous and highly conserved Ser/Thr kinase, is responsible for p47(phox) phosphorylation and that phosphorylation of p47(phox) by CK2 regulates the deactivation of NADPH oxidase.

METHODS

Here, we report that the residue Cys(196) of p47(phox) is a target of S-nitrosylation by S-nitrosothiol and peroxynitrite and that this modification enhanced phosphorylation of p47(phox) by CK2.

RESULTS

S-Nitrosylated p47(phox) enhanced CK2 b subunit binding, presumably due to alterations in protein conformation.

DISCUSSION

Taken together, we propose that S-nitrosylation of p47(phox) regulates the deactivation of NADPH oxidase via enhancement of p47(phox) phosphorylation by CK2.

摘要

目的

白细胞NADPH氧化酶在中性粒细胞中具有活性，是一种膜结合酶，它利用NADPH作为电子供体催化氧气还原为超氧阴离子（O2(-)）。此前，我们报道酪蛋白激酶2（CK2），一种普遍存在且高度保守的丝氨酸/苏氨酸激酶，负责p47(phox)的磷酸化，并且CK2对p47(phox)的磷酸化调节NADPH氧化酶的失活。

方法

在此，我们报道p47(phox)的半胱氨酸残基Cys(196)是亚硝基硫醇和过氧亚硝酸盐进行S-亚硝基化修饰的靶点，并且这种修饰增强了CK2对p47(phox)的磷酸化作用。

结果

S-亚硝基化的p47(phox)增强了CK2 β亚基的结合，推测这是由于蛋白质构象的改变。

讨论

综上所述，我们提出p47(phox)的S-亚硝基化通过增强CK2对p47(phox)的磷酸化来调节NADPH氧化酶的失活。