链脲佐菌素诱导的糖尿病大鼠胰腺的磁共振成像：钆弗塞胺P和钆喷酸葡胺。

Magnetic resonance imaging of the pancreas in streptozotocin-induced diabetic rats: Gadofluorine P and Gd-DOTA.

作者信息

Cho Hye Rim, Lee Youkyung, Doble Philip, Bishop David, Hare Dominic, Kim Young-Jae, Kim Kwang Gi, Jung Hye Seung, Park Kyong Soo, Choi Seung Hong, Moon Woo Kyung

机构信息

Hye Rim Cho, Youkyung Lee, Seung Hong Choi, Woo Kyung Moon, Department of Radiology, Seoul National University College of Medicine, Seoul 110-744, South Korea.

出版信息

World J Gastroenterol. 2015 May 21;21(19):5831-42. doi: 10.3748/wjg.v21.i19.5831.

DOI:10.3748/wjg.v21.i19.5831

PMID:26019447

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4438017/

Abstract

AIM

To investigate the performance of Gadofluorine P-enhanced magnetic resonance imaging (MRI) on the diagnosis of diabetes in a streptozotocin (STZ) -induced diabetic rat model.

METHODS

Fischer 344 rats were treated with STZ. Rats not treated with STZ served as controls. T1-weighted MRI was performed using a 3T scanner before and after the injection of Gd-DOTA or Gadofluorine P (6 diabetic rats, 5 controls). The normalized signal intensity (SI) and the enhancement ratio (ER) of the pancreas were measured at each time point, and the values were compared between the normal and diabetic rats using the Mann-Whitney test. In addition, the values were correlated with the mean islet number. Optimal cut-off values were calculated using a positive test based on receiver operating characteristics. Intrapancreatic Gd concentration after the injection of each contrast media was measured using laser ablation-inductively coupled plasma-mass spectrometry in a separate set of rats (4 diabetic rats, 4 controls for Gadofluorine P; 2, 2 for Gd-DOTA).

RESULTS

The normalized SI and ER of the pancreas using Gd-DOTA were not significantly different between diabetic rats and controls. With Gadofluorine P, the values were significantly higher in the diabetic rats than in the control rats 30 min after injection (P < 0.05). The area under the receiver operating characteristic curve that differentiated diabetic rats from the control group was greater for Gadofluorine P than for Gd-DOTA (0.967 vs 0.667, P = 0.085). An increase in normalized SI 30 min after Gadofluorine P was correlated with a decrease in the mean number of islets (r (2) = 0.510, P = 0.014). Intra-pancreatic Gd was higher in rats with Gadofluorine P injection than Gd-DOTA injection (Gadofluorine P vs Gd-DOTA, 7.37 vs 0.00, P < 0.01). A significant difference in the concentration of intrapancreatic Gd was observed between the control and diabetic animals that were sacrificed 30 min after Gadofluorine P injection (control vs diabetic, 3.25 ng/g vs 10.55 ng/g, P < 0.05) CONCLUSION: In this STZ-induced diabetes rat model, Gadofluorine P-enhanced MRI of the pancreas showed high accuracy in the diagnosis of diabetes.

摘要

目的

研究钆弗塞磷增强磁共振成像（MRI）在链脲佐菌素（STZ）诱导的糖尿病大鼠模型中对糖尿病的诊断性能。

方法

用STZ处理Fischer 344大鼠。未用STZ处理的大鼠作为对照。在注射钆双胺或钆弗塞磷之前和之后，使用3T扫描仪对6只糖尿病大鼠和5只对照大鼠进行T1加权MRI检查。在每个时间点测量胰腺的归一化信号强度（SI）和增强率（ER），并使用Mann-Whitney检验比较正常大鼠和糖尿病大鼠之间的值。此外，这些值与平均胰岛数量相关。使用基于受试者工作特征的阳性试验计算最佳截断值。在另一组大鼠（4只糖尿病大鼠，4只钆弗塞磷对照；2只、2只钆双胺对照）中，使用激光烧蚀-电感耦合等离子体质谱法测量注射每种造影剂后胰腺内的钆浓度。

结果

使用钆双胺时，糖尿病大鼠和对照大鼠胰腺的归一化SI和ER无显著差异。使用钆弗塞磷时，注射后30分钟糖尿病大鼠的值显著高于对照大鼠（P < 0.05）。钆弗塞磷区分糖尿病大鼠和对照组的受试者工作特征曲线下面积大于钆双胺（0.967对0.667，P = 0.085）。钆弗塞磷注射后30分钟归一化SI的增加与平均胰岛数量的减少相关（r (2) = 0.510，P = 0.014）。注射钆弗塞磷的大鼠胰腺内钆含量高于注射钆双胺的大鼠（钆弗塞磷对钆双胺，7.37对0.00，P < 0.01）。在注射钆弗塞磷后30分钟处死的对照动物和糖尿病动物之间，观察到胰腺内钆浓度有显著差异（对照对糖尿病，3.25 ng/g对10.55 ng/g，P < 0.05）。结论：在这个STZ诱导的糖尿病大鼠模型中，钆弗塞磷增强的胰腺MRI在糖尿病诊断中显示出高准确性。