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四种亲水性聚合物作为脂肪酶固定化载体的性能比较。

Comparison of the performances of four hydrophilic polymers as supports for lipase immobilisation.

作者信息

Toscano Lydia, Montero Gisela, Stoytcheva Margarita, Cervantes Lourdes, Gochev Velizar

机构信息

Institute of Engineering, Autonomous University of Baja California , Mexicali , Mexico ; Technological Institute of Mexicali , Mexicali , Mexico.

Institute of Engineering, Autonomous University of Baja California , Mexicali , Mexico.

出版信息

Biotechnol Biotechnol Equip. 2014 Jan 2;28(1):52-60. doi: 10.1080/13102818.2014.901684.

DOI:10.1080/13102818.2014.901684
PMID:26019488
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4433960/
Abstract

Four hydrophilic polymers in the form of beads - chitosan, alginate, alginate/polyvinyl alcohol (PVA), and chitosan-coated alginate - were used as supports for lipase immobilisation. Hydrogel beads were characterised by bead-size-distribution estimation, surface morphology studies, and polymer interactions assessment. Matrix performances - loading efficiency, immobilisation yield, enzyme activity, and stability retention - were evaluated and compared. Although the loading efficiency of the chitosan-coated Ca-alginate beads (79.8%) was inferior to that of the Ca-alginate (87%) and of the Ca-alginate/PVA beads (81.3%), their enzyme immobilisation yield (63.96%) was the most important. Moreover, lipase encapsulated in chitosan-coated Ca-alginate beads demonstrated better pH, thermal, and storage (89% residual activity after 30 days) stabilities. Immobilised lipase activity also increased in the order: alginate/PVA > chitosan > alginate > alginate/chitosan, and displayed a maximum at pH 8 and at temperatures of 45 °C (chitosan and Ca-alginate/PVA beads) and 50 °C (Ca-alginate and chitosan-coated Ca-alginate beads). Thus, chitosan-coated Ca-alginate beads could be considered as a suitable support for lipase immobilisation.

摘要

以珠状形式存在的四种亲水性聚合物——壳聚糖、海藻酸盐、海藻酸盐/聚乙烯醇（PVA）和壳聚糖包被的海藻酸盐——被用作固定化脂肪酶的载体。通过珠粒尺寸分布估计、表面形态研究和聚合物相互作用评估对水凝胶珠进行了表征。评估并比较了载体性能——负载效率、固定化产率、酶活性和稳定性保持率。尽管壳聚糖包被的钙海藻酸盐珠（79.8%）的负载效率低于钙海藻酸盐珠（87%）和钙海藻酸盐/PVA珠（81.3%），但其酶固定化产率（63.96%）却是最高的。此外，包封在壳聚糖包被的钙海藻酸盐珠中的脂肪酶表现出更好的pH稳定性、热稳定性和储存稳定性（30天后残留活性为89%）。固定化脂肪酶的活性也按以下顺序增加：海藻酸盐/PVA＞壳聚糖＞海藻酸盐＞海藻酸/壳聚糖，并且在pH 8以及45℃（壳聚糖和钙海藻酸盐/PVA珠）和50℃（钙海藻酸盐和壳聚糖包被的钙海藻酸盐珠）的温度下表现出最大值。因此，壳聚糖包被的钙海藻酸盐珠可被视为固定化脂肪酶的合适载体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/0d016bc3a098/tbeq-28-052-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/89b7cb9f895e/tbeq-28-052-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/cfc2ecf4a990/tbeq-28-052-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/7ad369b4b540/tbeq-28-052-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/9f98cafcfbb5/tbeq-28-052-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/0d016bc3a098/tbeq-28-052-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/89b7cb9f895e/tbeq-28-052-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/42f56daad10a/tbeq-28-052-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/44e9de574140/tbeq-28-052-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/ffe677ea1c38/tbeq-28-052-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/cfc2ecf4a990/tbeq-28-052-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/7ad369b4b540/tbeq-28-052-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/9f98cafcfbb5/tbeq-28-052-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e3e/4433960/0d016bc3a098/tbeq-28-052-g008.jpg

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