Department of Organic and Inorganic Chemistry, Laboratory of Biotechnology and Organic Synthesis (LABS), Federal University of Ceará, Campus do Pici, Fortaleza, Ceará, 60455-970, Brazil.
Federal Institute of Education, Science and Technology of Ceará, Campus de Crateús, Avenida Geraldo Barbosa Marques, 567, Crateús, CE, 63708-260, Brazil.
Bioprocess Biosyst Eng. 2021 Apr;44(4):785-792. doi: 10.1007/s00449-020-02487-2. Epub 2021 Jan 3.
Amano lipase AK from P. fluorescens was immobilized on different types of chitosan-containing supports. Chitosan lower molecular weight (2.5%), chitosan lower molecular weight/sodium alginate (2.5%/2.5%) and chitosan lower molecular weight/carrageenan (2.5%/2.5%) allowed the highest values of immobilization yields (IY) of 81, 81 and 83%, respectively. Best activity results were achieved using chitosan average molecular weight (5%) and chitosan lower molecular weight/sodium alginate (2.5%/2.5%) as support, with values of 1.40 and 1.30 U/g and with recovery activities of 45.75 and 35.6%, respectively. These derivatives were evaluated in the kinetic resolution of rac-indanol to obtain a key intermediate in the synthesis of a drug used in the treatment of Parkinson's disease. The most efficient derivatives in the kinetic resolution were lipase immobilized on chitosan average molecular weight (5.0%) and chitosan low molecular weight/sodium alginate, the latter leading to obtaining both (S)-indanol and (R)-indanyl acetate with > 99% ee and 50% conversion.
荧光假单胞菌脂肪酶 AK 被固定在不同类型的含壳聚糖的载体上。低分子量壳聚糖（2.5%）、低分子量壳聚糖/海藻酸钠（2.5%/2.5%）和低分子量壳聚糖/卡拉胶（2.5%/2.5%）允许最高的固定化产率（IY），分别为 81%、81%和 83%。使用平均分子量壳聚糖（5%）和低分子量壳聚糖/海藻酸钠（2.5%/2.5%）作为载体时，获得了最佳的酶活结果，酶活值分别为 1.40 和 1.30 U/g，回收率分别为 45.75%和 35.6%。这些衍生物在 rac-吲哚醇的动力学拆分中得到了评估，以获得一种用于治疗帕金森病的药物合成的关键中间体。在动力学拆分中最有效的衍生物是固定在平均分子量壳聚糖（5.0%）和低分子量壳聚糖/海藻酸钠上的脂肪酶，后者导致获得了（S）-吲哚醇和（R）-吲哚基乙酸酯，ee 值均>99%，转化率为 50%。
