Sakaridis Ioannis, Ganopoulos Ioannis, Madesis Panagiotis, Tsaftaris Athanasios, Argiriou Anagnostis
Institute of Applied Biosciences, Centre for Research and Technology Hellas , Thessaloniki , Greece.
Biotechnol Biotechnol Equip. 2014 Jan 2;28(1):107-111. doi: 10.1080/13102818.2014.901681.
An outbreak situation of human listeriosis requires a fast and accurate protocol for typing . Existing techniques are either characterized by low discriminatory power or are laborious and require several days to give a final result. Polymerase chain reaction (PCR) coupled with high resolution melting (HRM) analysis was investigated in this study as an alternative tool for a rapid and precise genotyping of isolates. Fifty-five isolates of isolated from poultry carcasses and the environment of four slaughterhouses were typed by HRM analysis using two specific markers, and genes. The analysis of genotype confidence percentage of isolates produced by HRM analysis generated dendrograms with two major groups and several subgroups. Furthermore, the analysis of the HRM curves revealed that all isolates could easily be distinguished. In conclusion, HRM was proven to be a fast and powerful tool for genotyping isolates of .
人类李斯特菌病的暴发情况需要一种快速准确的分型方案。现有技术要么鉴别能力低，要么费力且需要几天时间才能得出最终结果。本研究对聚合酶链反应（PCR）结合高分辨率熔解（HRM）分析作为一种对分离株进行快速精确基因分型的替代工具进行了研究。使用两个特异性标记物，即和基因，通过HRM分析对从四个屠宰场的家禽尸体和环境中分离出的55株分离株进行分型。对HRM分析产生的分离株基因型置信百分比的分析生成了具有两个主要组和几个亚组的树状图。此外，HRM曲线分析表明，所有分离株都能很容易地被区分。总之，HRM被证明是一种对分离株进行基因分型的快速且强大的工具。
