Department of Hygiene and Technology of Foods of Animal Origin, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
J Food Prot. 2011 Jun;74(6):1017-21. doi: 10.4315/0362-028X.JFP-10-545.
This study was conducted to determine the prevalence and antimicrobial resistance of Listeria monocytogenes recovered from chicken carcasses in slaughterhouses in Northern Greece. A total of 100 poultry samples (300 carcasses) were examined for Listeria spp. The samples were neck skin taken from four different slaughterhouses in Northern Greece. Forty samples were also taken from the environment of the slaughterhouses. Identification of L. monocytogenes was carried out by PCR and fingerprinting of the isolates by random amplified polymorphic DNA. L. monocytogenes strains isolated from chicken carcasses and from the environment of the slaughterhouses were also examined for antibiotic resistance. Fifty-five isolates of L. monocytogenes were tested for susceptibility to 20 antibiotics using the disk diffusion method. Listeria spp. were present in 99 of the poultry samples tested (99%), and 38 yielded L monocytogenes (38%). L. monocytogenes was also isolated in 80% of samples from the environment of a certain slaughterhouse, while the other slaughterhouses were found to be contaminated only with Listeria spp. All isolates were resistant to nalidixic acid and oxolinic acid, the majority of them to clindamycin, and only a few to tetracycline and oxytetracycline, whereas they were found to be susceptible to all other antimicrobials. The results of this study demonstrate a high prevalence of L. monocytogenes contamination in chicken carcasses, and all isolates were found to be sensitive to the antimicrobials most commonly used to treat human listeriosis.
本研究旨在确定希腊北部屠宰场鸡胴体中分离出的李斯特菌的流行率和抗药性。对 100 份家禽样本（300 只胴体）进行了李斯特菌属检测。这些样本是从希腊北部四个不同的屠宰场采集的颈部皮肤。还从屠宰场的环境中采集了 40 个样本。通过 PCR 鉴定李斯特菌，通过随机扩增多态性 DNA 对分离株进行指纹分析。对从鸡胴体和屠宰场环境中分离出的李斯特菌菌株进行了抗生素耐药性检测。使用纸片扩散法对 55 株李斯特菌进行了 20 种抗生素的药敏试验。在所检测的 99%的家禽样本中（99 份）存在李斯特菌属，其中 38 份样本产单核细胞李斯特菌（38%）。在某个屠宰场环境的样本中，80%分离出了李斯特菌属，而其他屠宰场仅被发现污染有李斯特菌属。所有分离株均对萘啶酸和恶喹酸耐药，其中大多数对克林霉素耐药，只有少数对四环素和土霉素耐药，而对所有其他抗菌药物均敏感。本研究结果表明，鸡胴体中李斯特菌属污染的流行率很高，所有分离株对治疗人类李斯特菌病最常用的抗生素均敏感。
