Li Chen, Liu Bolin
Sheng Wu Gong Cheng Xue Bao. 2015 Jan;31(1):53-64.
Insecticidal protein genes from Bacillus thuringiensis are currently the most widely used insect-resistant genes. They have been transferred to many crops for breeding and production. Among them, cotton, maize, potato and other insect-resistant crops are commercialized, creating considerable economic benefit. In this review, we summarized advances in identifying functional genes and transgenic crops for insect resistance, compared different strategies for enhancing vigor of insecticidal protein and utilizing gene stacking as well as listing valuable groups of stacked genes. In addition, the methods for multiple gene transformation was discussed.
苏云金芽孢杆菌的杀虫蛋白基因是目前应用最为广泛的抗虫基因。这些基因已被转入多种作物用于育种和生产。其中，棉花、玉米、马铃薯等抗虫作物已实现商业化，创造了可观的经济效益。在本综述中，我们总结了抗虫功能基因鉴定及转基因作物方面的进展，比较了增强杀虫蛋白活力及利用基因叠加的不同策略，并列举了有价值的叠加基因组合。此外，还讨论了多基因转化方法。
