Bachin Dmitry, Nazarenko Lyudmila V, Mironov Kirill S, Pisareva Tatiana, Allakhverdiev Suleyman I, Los Dmitry A
Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya street 35, 127276 Moscow, Russia.
Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya street 35, 127276 Moscow, Russia Moscow City Teacher Training University, 2nd Agricultural Passage 4, 129226 Moscow, Russia.
FEMS Microbiol Lett. 2015 Jun;362(12):fnv090. doi: 10.1093/femsle/fnv090. Epub 2015 May 27.
Calcium plays an essential role in a variety of stress responses of eukaryotic cells; however, its function in prokaryotes is obscure. Bacterial ion channels that transport Ca(2+) are barely known. We investigated temperature-induced changes in intracellular concentration of Ca(2+), Na(+) and K(+) in the cyanobacterium Synechocystis sp. strain PCC 6803 and its mutant that is defective in mechanosensitive ion channel MscL. Concentration of cations rapidly and transiently increased in wild-type cells in response to cold and heat treatments. These changes in ionic concentrations correlated with the changes in cytoplasmic volume that transiently decreased in response to temperature treatments. However, no increase in ionic concentrations was observed in the MscL-mutant cells. It implies that MscL functions as a non-specific ion channel, and it participates in regulation of cell volume under temperature-stress conditions.
钙在真核细胞的多种应激反应中起着至关重要的作用；然而，其在原核生物中的功能尚不清楚。几乎没有已知的能转运Ca(2+)的细菌离子通道。我们研究了集胞藻PCC 6803及其机械敏感离子通道MscL有缺陷的突变体中，温度诱导的Ca(2+)、Na(+)和K(+)细胞内浓度变化。在野生型细胞中，冷处理和热处理会使阳离子浓度迅速且短暂地升高。这些离子浓度的变化与细胞质体积的变化相关，细胞质体积会因温度处理而短暂减小。然而，在MscL突变体细胞中未观察到离子浓度增加。这意味着MscL作为一种非特异性离子通道发挥作用，并参与温度应激条件下的细胞体积调节。
