Prakash Jogadhenu S S, Sinetova Maria, Zorina Anna, Kupriyanova Elena, Suzuki Iwane, Murata Norio, Los Dmitry A
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Gachibowli, Hyderbad 500 046, Andhra Pradesh, India.
Mol Biosyst. 2009 Dec;5(12):1904-12. doi: 10.1039/B903022k. Epub 2009 Sep 10.
Changes in the supercoiling of genomic DNA play an important role in the regulation of gene expression. We compared the genome-wide expression of genes in cells of the cyanobacterium Synechocystis sp. PCC 6803 when they were subjected to salt, cold, and heat stress, in the presence of novobiocin, an inhibitor of DNA gyrase, and in its absence. The analysis revealed that the expression of a large number of stress-inducible genes depends on the extent of genomic DNA supercoiling. The function of the two-component regulatory systems, which are known as sensors and transducers of salt, cold, and heat stress, depends on, and might be controlled by, the degree of supercoiling of the genomic DNA. These results suggest that stress-induced changes in superhelicity of genomic DNA provide an important permissive background for successful acclimatization of cyanobacterial cells to stress conditions.
基因组DNA超螺旋结构的变化在基因表达调控中起着重要作用。我们比较了集胞藻PCC 6803细胞在盐胁迫、冷胁迫和热胁迫下，以及在DNA回旋酶抑制剂新生霉素存在和不存在的情况下，全基因组基因的表达情况。分析表明，大量应激诱导基因的表达取决于基因组DNA超螺旋的程度。作为盐、冷和热应激的传感器和转导器的双组分调节系统的功能取决于基因组DNA的超螺旋程度，并且可能受其控制。这些结果表明，应激诱导的基因组DNA超螺旋变化为蓝藻细胞成功适应应激条件提供了重要的许可背景。
