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人脂肪来源的间充质祖细胞植入兔关节软骨。

Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

作者信息

Wang Wen, He Na, Feng Chenchen, Liu Victor, Zhang Luyi, Wang Fei, He Jiaping, Zhu Tengfang, Wang Shuyang, Qiao Weiwei, Li Suke, Zhou Guangdong, Zhang Li, Dai Chengxiang, Cao Wei

机构信息

Cellular Biomedicine Group, Palo Alto, CA 94301, USA.

Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200433, China.

出版信息

Int J Mol Sci. 2015 May 27;16(6):12076-91. doi: 10.3390/ijms160612076.

DOI:10.3390/ijms160612076
PMID:26023716
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4490430/
Abstract

Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

摘要

间充质干细胞（MSCs）已知具有关节软骨再生的潜力，并被建议用于治疗骨关节炎（OA）。在此，我们研究了关节腔内注射异种来源的人脂肪间充质祖细胞（haMPCs）是否能促进兔OA模型中的关节软骨修复并植入兔关节软骨。将haMPCs进行体外培养，并评估细胞的表型和分化特征。通过手术切断前交叉韧带（ACLT）和切除膝关节内侧半月板诱导OA。术后六周，将透明质酸（HA）或haMPCs注入膝关节，对侧膝关节作为正常对照。所有动物在术后第16周处死。通过宏观检查、苏木精/伊红（HE）染色、番红O/固绿染色和免疫组织化学进行评估。数据表明，haMPC治疗促进了软骨修复。在haMPC治疗的软骨中可直接检测到人类线粒体信号。haMPCs在体内表达人类白细胞抗原I（HLA-I）但不表达HLA-II-DR。这些结果表明，关节腔内注射haMPCs可促进兔OA模型中关节软骨的再生，并支持MPCs可在HLA不相容个体之间移植的观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dcf/4490430/a0848b428009/ijms-16-12076-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dcf/4490430/3f945b493c06/ijms-16-12076-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dcf/4490430/77fe49c98597/ijms-16-12076-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dcf/4490430/85e8dd0e59f1/ijms-16-12076-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dcf/4490430/96ef4db37f02/ijms-16-12076-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3dcf/4490430/81607845d15a/ijms-16-12076-g005.jpg
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