Wang Guang-Peng, Liu Wen-Jie, Si Gui-Cai, Hu Guo-Wen
a Key Laboratory of Alpine Ecology and Biodiversity , Institute of Tibetan Plateau Research, Chinese Academy of Sciences , Beijing , China .
b State Key Laboratory of Cryospheric Sciences , Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences , Lanzhou , China .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2570-1. doi: 10.3109/19401736.2015.1038808. Epub 2015 May 29.
Trichopodus leerii has been given many popular names in the ornament market, such as pearl gourami, lace gourami and mosaic gourami, which causes confusion in species identification. This species belongs in the family Osphronemidae of Perciformes. This species and its congeners are characterized by a brownish-silver body, covered in a pearl-like pattern. In this study, we first determined and described the complete mitogenome sequence of T. leerii, which is 16,472 bp in length. The overall base composition is 29.2%, 27.3%, 28.0% and 15.5% for A, C, T and G, respectively, with a slight bias in the AT content (57.2%). All protein-coding genes share the start codon ATG and most of them have TAA or TAG as the stop codon, except ND4 and ND6 use an incomplete stop codon T. Maximum likelihood tree and Bayesian analyses based on partitioned nucleotide sequences of 12 mitochondrial protein-coding genes were constructed, and both yielded identical topologies. These results are expected to provide useful molecular data for species identification and further phylogenetic studies of Osphronemidae and Perciformes.
李氏丝足鲈在观赏鱼市场有很多俗称,如珍珠马甲、花边马甲和马赛克马甲,这导致了物种鉴定的混乱。该物种属于鲈形目丝足鲈科。该物种及其同属物种的特征是身体呈褐银色,覆盖着珍珠状图案。在本研究中,我们首次测定并描述了李氏丝足鲈的完整线粒体基因组序列,其长度为16,472 bp。总体碱基组成为A占29.2%、C占27.3%、T占28.0%、G占15.5%,AT含量略有偏向(57.2%)。所有蛋白质编码基因都以ATG作为起始密码子,除了ND4和ND6使用不完全终止密码子T外,大多数基因以TAA或TAG作为终止密码子。基于12个线粒体蛋白质编码基因的分区核苷酸序列构建了最大似然树和贝叶斯分析,两者产生了相同的拓扑结构。这些结果有望为丝足鲈科和鲈形目的物种鉴定和进一步的系统发育研究提供有用的分子数据。