Jang Yo-Soon, Lee Eun Kyung, Park Jeong-Ho, Kim Ki-Yong, Kim Sung
a East Sea Research Institute, Korea Institute of Ocean Science & Technology , Hujung-ri, Jukbyeon-myun, Uljin-gun , Gyeongbuk , Republic of Korea.
b Department of Marine Biotechnology , Korea University of Science & Technology , Daejeon , Republic of Korea.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4041-4043. doi: 10.3109/19401736.2014.1003834. Epub 2015 Jan 28.
Sebastes is a diverse genus of fish in the family Scorpaenidae. The larvae and adults in this genus have similar morphological characteristics. To obtain basic information for their identification, the mitogenome of S. steindachneri was sequenced using next-generation sequencing. The mitogenome was 16,450 bp in length, and comprised of 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNAs, and a control region. The mitogenome is 27.4% A, 21.1% T, 17.5% G, and 28.0% C, showing a slight AT bias (54.5%). ATG was the start codon in all of the PCGs, except COX1 (GTG). The termination codon in the PCGs was TAA, except in ND1 and ND3 (TAG), ND4 (AGA), and Cytb (incomplete termination codon, T). All of the tRNAs had a typical cloverleaf shape, except tRNA, which lacked the DHU arm. The mitogenome of S. steindachneri should be useful for identifying larvae and constructing a phylogenetic structure for the Scorpaenidae.
鲈鲉属是鲉科中一个多样的鱼类属。该属的幼体和成体具有相似的形态特征。为了获取其鉴定的基础信息，使用二代测序技术对施氏鲈鲉的线粒体基因组进行了测序。线粒体基因组长度为16450 bp，由13个蛋白质编码基因（PCGs）、2个rRNA、22个tRNA和一个控制区组成。线粒体基因组中A占27.4%，T占21.1%，G占17.5%，C占28.0%，显示出轻微的AT偏向性（54.5%）。除COX1（GTG）外，所有PCGs的起始密码子均为ATG。PCGs中的终止密码子除ND1和ND3为TAG、ND4为AGA以及Cytb为不完全终止密码子T外，其余均为TAA。除缺少DHU臂的tRNA外，所有tRNA均具有典型的三叶草形状。施氏鲈鲉的线粒体基因组应有助于幼体鉴定以及构建鲉科的系统发育结构。
