Department of Obstetrics and Gynecology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Medical Centre for Experiment and Testing, Capital Medical University, Beijing, China.
Diabetes Res Clin Pract. 2015 Aug;109(2):404-10. doi: 10.1016/j.diabres.2015.05.010. Epub 2015 May 12.
The relationship between oxidative stress and the International Association of Diabetes and Pregnancy Study Groups (IADPSG) criteria diagnosed gestational diabetes mellitus GDM isn't well known. Our aim is to evaluate the oxidative stress in women diagnosed by the IADPSG criteria versus the American Diabetes Association (ADA) criteria in China.
Malondialdehyde (MDA), 8-isoprostane (8IsoP), xanthine oxidize (XO), lipid peroxides (LPO), superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), vitamin C (Vc) and vitamins E (Ve) were determined in maternal and cord plasma and placenta of 68 pregnant women. Among these, 28 were diagnosed with GDM while the other 40 were controls.
The maternal, cord and placental MDA, XO and 8IsoP levels were significantly higher while SOD and TAC levels were significantly lower in GDM women by either criterion (P < 0.05). XO and 8IsoP levels were higher in ADA group than IADPSG only group while TAC levels significant lower (P < 0.05). Cord MDA, cord and placental XO, and maternal and cord 8IsoP showed significant positive relationship with HbA1c values (P < 0.05). Cord XO levels increased (P < 0.05) while maternal and placental SOD levels decreased (P < 0.05) in women who received cesarean section compared with those delivered normally. Increased XO levels and decreased Ve levels in cord plasma were also found in macrosomia (P < 0.05).
Oxidative stress is present in women diagnosed by IADPSG but to a lesser degree than by ADA. All these women should be monitored and perhaps antioxidant supplemented.
国际妊娠合并糖尿病研究组织（IADPSG）标准与美国糖尿病协会（ADA）标准诊断的妊娠期糖尿病（GDM）之间的氧化应激关系尚不清楚。本研究旨在评估中国 IADPSG 与 ADA 标准诊断的 GDM 妇女的氧化应激情况。
测定 68 例孕妇母血、脐血和胎盘组织中丙二醛（MDA）、8-异前列腺素（8IsoP）、黄嘌呤氧化酶（XO）、脂质过氧化物（LPO）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）、总抗氧化能力（TAC）、维生素 C（Vc）和维生素 E（Ve）。其中 28 例诊断为 GDM，40 例为对照组。
两种标准诊断的 GDM 患者母血、脐血和胎盘组织 MDA、XO 和 8IsoP 水平显著升高，SOD 和 TAC 水平显著降低（P<0.05）。ADA 组的 XO 和 8IsoP 水平高于 IADPSG 组，而 TAC 水平显著降低（P<0.05）。脐血 MDA、脐血和胎盘 XO 以及母血和脐血 8IsoP 与 HbA1c 值呈显著正相关（P<0.05）。与正常分娩相比，剖宫产孕妇的脐血 XO 水平升高（P<0.05），母血和胎盘 SOD 水平降低（P<0.05）。巨大儿孕妇的脐血 XO 水平升高（P<0.05）和 Ve 水平降低（P<0.05）。
IADPSG 诊断的 GDM 患者存在氧化应激，但程度低于 ADA 标准。所有这些妇女都应进行监测，或许需要补充抗氧化剂。
