Farahani Roya Kishani, Azimzadeh Pedram, Rostami Elham, Malekpour Habib, Aghdae Hamid Asadzadeh, Pourhoseingholi Mohamad Amin, Nazemalhosseini Mojarad Ehsan, Zali Mohammad Reza
Basic and Molecular Epidemiology of Gastroenterology, Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran E-mail :
Asian Pac J Cancer Prev. 2015;16(10):4215-8. doi: 10.7314/apjcp.2015.16.10.4215.
Gastric cancer (GC) is one of the most common malignancies in the world. It is the first cause of cancer deaths in both sexes In Iranian population. Circulating insulin-like growth factor-one (IGF-1) levels have been associated for gastric cancer. IGF-1 protein has central roles involved in the regulation of epithelial cell growth, proliferation, transformation, apoptosis and metastasis. Single nucleotide polymorphism in IGF-1 regulatory elements may lead to alter in IGF-1 expression level and GC susceptibility. The aim of this study was to investigate the influence of IGF-1 gene polymorphism (rs5742612) on risk of GC and clinicopathological features for the first time in Iranian population. In total, 241 subjects including 100 patients with GC and 141 healthy controls were recruited in our study. Genotypes were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with DNA from peripheral blood. The polymorphism was statistically analyzed to investigate the relationship with the risk of GC and clinicopathological properties. Logistic regression analysis revealed that there was no significant association between rs5742612 and the risk of GC. In addition, no significant association between genotypes and clinicopathological features was observed (p value>0.05). The frequencies of the CC, CT, and TT genotypes were 97%, 3%, and 0%, respectively, among the cases, and 97.9%, 2.1%, and 0%, respectively, among the controls. CC genotype was more frequent in cases and controls. The frequencies of C and T alleles were 98.9% and 1.1% in controls and 98.5% and 1.5% in patient respectively. Our results provide the first evidence that this variant is rare in Iranian population and it may not be a powerful genetic predisposing biomarker for prediction GC clinicopathological features in an Iranian population.
胃癌（GC）是世界上最常见的恶性肿瘤之一。在伊朗人群中，它是男女癌症死亡的首要原因。循环胰岛素样生长因子-1（IGF-1）水平与胃癌有关。IGF-1蛋白在调节上皮细胞生长、增殖、转化、凋亡和转移中起核心作用。IGF-1调控元件中的单核苷酸多态性可能导致IGF-1表达水平改变和GC易感性。本研究的目的是首次在伊朗人群中调查IGF-1基因多态性（rs5742612）对GC风险和临床病理特征的影响。本研究共招募了241名受试者，包括100名GC患者和141名健康对照。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析法，以外周血DNA分析基因型。对多态性进行统计学分析，以研究其与GC风险和临床病理特征的关系。逻辑回归分析显示，rs5742612与GC风险之间无显著关联。此外，未观察到基因型与临床病理特征之间存在显著关联（p值>0.05）。病例组中CC、CT和TT基因型的频率分别为97%、3%和0%，对照组中分别为97.9%、2.1%和0%。CC基因型在病例组和对照组中更为常见。对照组中C和T等位基因的频率分别为98.9%和1.1%，患者组中分别为98.5%和1.5%。我们的结果首次证明，该变体在伊朗人群中罕见，它可能不是预测伊朗人群GC临床病理特征的有力遗传易感性生物标志物。
