Vollmar B, Waldner H, Schmand J, Conzen P F, Goetz A E, Habazettl H, Schweiberer L, Brendel W
Dept. of Surgery, Innenstad, Ludwigs Maximilians University of Munich, FRG.
Scand J Gastroenterol. 1989 Dec;24(10):1253-64. doi: 10.3109/00365528909090796.
The pancreatic release of arachidonic acid metabolites was studied in a porcine model of acute pancreatitis. In situ isolation of the pancreatic gland enabled selective collection of pancreatic venous blood, pancreatic lymph, and ascites fluid. Three experimental groups were studied: 1) control (n = 9); 2) hemorrhagic pancreatitis induced by injection of 5% bile salt (sodium taurocholate) into the pancreatic duct (n = 10); and 3) edematous pancreatitis induced by injection of free fatty acid (FFA) into the pancreatic artery (n = 10). Determinations of cyclooxygenase metabolites were performed by radioimmunoassay; lipoxygenase metabolites (LTC4, LTD4) were measured by radioimmunoassay after purification by high-performance liquid chromatography. Prostaglandin (PG)F1 alpha, thromboxane B2, and PGF2 alpha concentrations were almost doubled in the lymph of the FFA group during pancreatitis, as were PGF1 alpha levels in pancreatic venous blood. However, concentrations of cyclooxygenase metabolites remained unchanged in the control group and in the bile salt group. Concentrations of LTC4 and LTD4 in lymph and ascites fluid of both pancreatitis groups increased from about 50 pg/ml to a mean level of 600 pg/ml at 6 h. Leukotriene concentrations in the control group were consistently below 50 pg/ml. The results of this study indicate that above all LTC4 and LTD4 are released from the organ and that these arachidonic acid metabolites may be also involved in the events following acute pancreatitis contributing to the systemic effects of the disease.
在急性胰腺炎的猪模型中研究了胰腺中花生四烯酸代谢产物的释放情况。胰腺原位分离可选择性收集胰腺静脉血、胰腺淋巴液和腹水。研究了三个实验组：1）对照组（n = 9）；2）通过向胰管注射5%胆盐（牛磺胆酸钠）诱导出血性胰腺炎（n = 10）；3）通过向胰腺动脉注射游离脂肪酸（FFA）诱导水肿性胰腺炎（n = 10）。通过放射免疫测定法测定环氧化酶代谢产物；脂氧合酶代谢产物（LTC4、LTD4）在通过高效液相色谱法纯化后通过放射免疫测定法进行测量。在胰腺炎期间，FFA组淋巴液中的前列腺素（PG）F1α、血栓素B2和PGF2α浓度几乎增加了一倍，胰腺静脉血中的PGF1α水平也是如此。然而，对照组和胆盐组中环氧化酶代谢产物的浓度保持不变。两个胰腺炎组的淋巴液和腹水中LTC4和LTD4的浓度在6小时时从约50 pg/ml增加到平均600 pg/ml水平。对照组中的白三烯浓度始终低于50 pg/ml。本研究结果表明，最重要的是LTC4和LTD4从该器官释放，并且这些花生四烯酸代谢产物可能也参与急性胰腺炎后的事件，对该疾病的全身效应有影响。
