Sharma Neha, Tripathi Anita, Sanan-Mishra Neeti
Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology , New Delhi, India.
Front Plant Sci. 2015 May 13;6:333. doi: 10.3389/fpls.2015.00333. eCollection 2015.
Plant microRNAs (miRs) have emerged as important regulators of gene expression under normal as well as stressful environments. Rice is an important cereal crop whose productivity is compromised due to various abiotic stress factors such as salt, heat and drought. In the present study, we have investigated the role of rice-specific Osa-miR820, in indica rice cultivars showing contrasting response to salt stress. The dissection of expression patterns indicated that the miR is present in all the tissues but is enriched in the anther tissues. In salinity, the miR levels are up-regulated in the leaf tissues but down-regulated in the root tissues. To map the deregulation under salt stress comprehensive time kinetics of expression was performed in the leaf and root tissues. The reproductive stages were also analyzed under salt stress. It emerged that a common regulatory scheme for Osa-miR820 expression is present in the salt-susceptible Pusa Basmati 1 and salt-tolerant Pokkali varieties, although there is a variation in the levels of the miR and its target transcript, OsDRM2. The regulation of Osa-miR820 and its target were also studied under other abiotic stresses. This study thus captures the window for the miR-target correlation and the putative role of this regulation is discussed. This will help in gaining useful insights on the role of species specific miRs in plant development and abiotic stress response.
植物微小RNA（miR）已成为正常及胁迫环境下基因表达的重要调节因子。水稻是一种重要的谷类作物，其生产力因盐、热和干旱等各种非生物胁迫因素而受到影响。在本研究中，我们调查了水稻特有的Osa-miR820在对盐胁迫表现出不同反应的籼稻品种中的作用。对表达模式的剖析表明，该miR存在于所有组织中，但在花药组织中富集。在盐胁迫下，叶片组织中miR水平上调，而根组织中则下调。为了绘制盐胁迫下的失调图谱，在叶片和根组织中进行了全面的表达时间动力学研究。还对生殖阶段进行了盐胁迫分析。结果表明，在盐敏感的Pusa Basmati 1和耐盐的Pokkali品种中，存在Osa-miR820表达的共同调控机制，尽管miR及其靶转录本OsDRM2的水平存在差异。还研究了其他非生物胁迫下Osa-miR820及其靶标的调控。因此，本研究捕捉到了miR-靶标相关性的窗口，并讨论了这种调控的推定作用。这将有助于深入了解物种特异性miR在植物发育和非生物胁迫反应中的作用。
