Zheng Lu, Cong Hai-jian, Wu Bin, Xue Ming, Xiang Ting, Yao Zhong-qing, Lin Wen-hui
Yangtze River Pharmaceutical Group Co., Ltd., Taizhou 225321, People's Republic of China Shanghai Haini Pharmaceutical Co., Ltd., Yangtze River Pharmaceutical Group, Shanghai 201318, People's Republic of China.
Shanghai Haini Pharmaceutical Co., Ltd., Yangtze River Pharmaceutical Group, Shanghai 201318, People's Republic of China.
J Chromatogr Sci. 2015 Oct;53(9):1562-9. doi: 10.1093/chromsci/bmv058. Epub 2015 Jun 1.
A high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry method was established to detect as many constituents in rat biological fluids as possible after oral administration of Qing Ru Xiao granules (QRX). An Agilent Poroshell 120 EC-C18 column was adopted to separate the sample constituents, and mass spectra were acquired in positive and negative ion modes. First, the fingerprints of QRX were established, resulting in 28 components being detected within 30 min. Among these compounds, 11 were tentatively identified by comparing the retention times and mass spectral data with those of reference standards and the reference literature; the other 17 components were tentatively assigned solely based on the mass spectrometry data. Furthermore, metabolites in rat plasma and urine after oral administration of QRX were also analyzed. A total of 15 compounds were identified, including 13 prototypes and 2 metabolites through metabolic pathways of oxidation. This is the first systematic study on the metabolic profiling of QRX.
建立了一种高效液相色谱/四极杆飞行时间质谱法，用于在大鼠口服清乳消颗粒（QRX）后尽可能多地检测其生物体液中的成分。采用安捷伦Poroshell 120 EC-C18色谱柱分离样品成分，并在正离子和负离子模式下采集质谱。首先，建立了QRX的指纹图谱，在30分钟内检测到28种成分。在这些化合物中，通过将保留时间和质谱数据与参考标准品和参考文献进行比较，初步鉴定出11种；另外17种成分仅根据质谱数据进行了初步归属。此外，还分析了大鼠口服QRX后血浆和尿液中的代谢产物。共鉴定出15种化合物，包括13种原型成分和2种通过氧化代谢途径产生的代谢产物。这是对QRX代谢谱的首次系统研究。
