Zheng Lu, Cong Haijian, Xue Ming, Xiang Ting, Yao Zhongqing, Wu Bin, Lin Wenhui
Yangtze River Pharmaceutical Group, Taizhou, 225321, People's Republic of China.
Biomed Chromatogr. 2015 Jan;29(1):75-86. doi: 10.1002/bmc.3242. Epub 2014 Jun 11.
A high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry method was established to detect as many constituents in rat biological fluids as possible after oral administration of Shuanghua Baihe tablets (SBT). An Agilent Poroshell 120 EC-C18 column was adopted to separate the samples, and mass spectra were acquired in positive and negative modes. First, the fingerprints of SBT were established, resulting in 32 components being detected within 40 min. Among these compounds, 12 were tentatively identified by comparing the retention times and mass spectral data with those of reference standards and the reference literature; the other 20 components were tentatively assigned solely based on the MS data. Furthermore, metabolites in rat plasma and urine after oral administration of SBT were also analyzed. A total of 19 compounds were identified, including 13 prototypes and six metabolites through metabolic pathways of demethylation and glucuronide conjugation. Glucuronidated alkaloids were the main constituents in the plasma, and were then excreted from urine. This is the first systematic study on the metabolic profiling of SBT.
建立了一种高效液相色谱/四极杆飞行时间质谱法，用于检测大鼠口服双花百合片（SBT）后生物体液中的尽可能多的成分。采用安捷伦Poroshell 120 EC-C18柱分离样品，并在正、负离子模式下采集质谱。首先，建立了SBT的指纹图谱，在40分钟内检测到32个成分。在这些化合物中，通过将保留时间和质谱数据与参考标准品和参考文献进行比较，初步鉴定出12个；另外20个成分仅根据质谱数据进行了初步归属。此外，还分析了大鼠口服SBT后血浆和尿液中的代谢产物。共鉴定出19种化合物，包括13种原型成分和6种通过去甲基化和葡萄糖醛酸结合代谢途径产生的代谢产物。葡萄糖醛酸化生物碱是血浆中的主要成分，随后从尿液中排出。这是首次对SBT进行代谢谱的系统研究。
