Ikeda Kazushi, Ito Akira, Sato Masanori, Kanno Shota, Kawabe Yoshinori, Kamihira Masamichi
Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.
Department of Chemical Engineering, Kyushu University, Fukuoka, Japan.
J Tissue Eng Regen Med. 2017 May;11(5):1322-1331. doi: 10.1002/term.2030. Epub 2015 Jun 1.
Although skeletal muscle tissue engineering has been extensively studied, the physical forces produced by tissue-engineered skeletal muscles remain to be improved for potential clinical utility. In this study, we examined the effects of mild heat stimulation and supplementation of a l-ascorbic acid derivative, l-ascorbic acid 2-phosphate (AscP), on myoblast differentiation and physical force generation of tissue-engineered skeletal muscles. Compared with control cultures at 37°C, mouse C2C12 myoblast cells cultured at 39°C enhanced myotube diameter (skeletal muscle hypertrophy), whereas mild heat stimulation did not promote myotube formation (differentiation rate). Conversely, AscP supplementation resulted in an increased differentiation rate but did not induce skeletal muscle hypertrophy. Following combined treatment with mild heat stimulation and AscP supplementation, both skeletal muscle hypertrophy and differentiation rate were enhanced. Moreover, the active tension produced by the tissue-engineered skeletal muscles was improved following combined treatment. These findings indicate that tissue culture using mild heat stimulation and AscP supplementation is a promising approach to enhance the function of tissue-engineered skeletal muscles. Copyright © 2015 John Wiley & Sons, Ltd.
尽管骨骼肌组织工程已得到广泛研究，但组织工程化骨骼肌产生的物理力仍有待改善以实现潜在的临床应用。在本研究中，我们研究了温和热刺激和添加L - 抗坏血酸衍生物L - 抗坏血酸2 - 磷酸酯（AscP）对成肌细胞分化和组织工程化骨骼肌物理力产生的影响。与在37°C培养的对照培养物相比，在39°C培养的小鼠C2C12成肌细胞增强了肌管直径（骨骼肌肥大），而温和热刺激并未促进肌管形成（分化率）。相反，添加AscP导致分化率增加，但未诱导骨骼肌肥大。在温和热刺激和添加AscP联合处理后，骨骼肌肥大和分化率均得到增强。此外，联合处理后组织工程化骨骼肌产生的主动张力得到改善。这些发现表明，使用温和热刺激和添加AscP的组织培养是增强组织工程化骨骼肌功能的一种有前景的方法。版权所有© 2015约翰威立父子有限公司。
