Zanella Bruna Tereza Thomazini, Magiore Isabele Cristina, Duran Bruno Oliveira Silva, Pereira Guilherme Gutierrez, Vicente Igor Simões Tiagua, Carvalho Pedro Luiz Pucci Figueiredo, Salomão Rondinelle Artur Simões, Mareco Edson Assunção, Carvalho Robson Francisco, Paula Tassiana Gutierrez de, Barros Margarida Maria, Dal-Pai-Silva Maeli
Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University, UNESP, Botucatu 18618-689, São Paulo, Brazil.
Department of Histology, Embryology and Cell Biology, Institute of Biological Sciences, Federal University of Goiás (UFG), Goiânia 74690-900, Goiás, Brazil.
Int J Mol Sci. 2021 Mar 15;22(6):2995. doi: 10.3390/ijms22062995.
In fish, fasting leads to loss of muscle mass. This condition triggers oxidative stress, and therefore, antioxidants can be an alternative to muscle recovery. We investigated the effects of antioxidant ascorbic acid (AA) on the morphology, antioxidant enzyme activity, and gene expression in the skeletal muscle of pacu () following fasting, using in vitro and in vivo strategies. Isolated muscle cells of the pacu were subjected to 72 h of nutrient restriction, followed by 24 h of incubation with nutrients or nutrients and AA (200 µM). Fish were fasted for 15 days, followed by 6 h and 15 and 30 days of refeeding with 100, 200, and 400 mg/kg of AA supplementation. AA addition increased cell diameter and the expression of anabolic and cell proliferation genes in vitro. In vivo, 400 mg/kg of AA increased anabolic and proliferative genes expression at 6 h of refeeding, the fiber diameter and the expression of genes related to cell proliferation at 15 days, and the expression of catabolic and oxidative metabolism genes at 30 days. Catalase activity remained low in the higher supplementation group. In conclusion, AA directly affected the isolated muscle cells, and the higher AA supplementation positively influenced muscle growth after fasting.
在鱼类中，禁食会导致肌肉质量下降。这种情况会引发氧化应激，因此，抗氧化剂可能是促进肌肉恢复的一种选择。我们采用体外和体内实验方法，研究了抗氧化剂抗坏血酸（AA）对禁食后的淡水白鲳（）骨骼肌形态、抗氧化酶活性和基因表达的影响。将分离出的淡水白鲳肌肉细胞进行72小时的营养限制，然后分别用营养物质或营养物质与AA（200μM）孵育24小时。将鱼禁食15天，随后分别在再投喂100、200和400mg/kg AA的情况下，再投喂6小时、15天和30天。在体外，添加AA可增加细胞直径以及合成代谢和细胞增殖基因的表达。在体内，400mg/kg的AA在再投喂6小时时可增加合成代谢和增殖基因的表达，在15天时可增加纤维直径以及与细胞增殖相关的基因表达，在30天时可增加分解代谢和氧化代谢基因的表达。在高剂量补充组中，过氧化氢酶活性仍然较低。总之，AA直接影响分离的肌肉细胞，较高剂量的AA补充对禁食后的肌肉生长有积极影响。
