São Paulo State University (UNESP), Institute of Biosciences, Department of Morphology, Botucatu, São Paulo, Brazil.
University of West São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil.
Sci Rep. 2019 Feb 18;9(1):2229. doi: 10.1038/s41598-019-38536-4.
The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth.
鱼类胚胎后骨骼肌肉的生长涉及成肌细胞的增殖、迁移和分化，包括胚胎成肌发生的主要事件。抗坏血酸作为一种抗氧化剂，在细胞和生化水平上发挥着重要作用，有助于适当的胶原蛋白生物合成，这对于连接组织和骨骼组织的结构是必需的。然而，抗坏血酸是否能直接影响鱼类的成肌发生和骨骼肌肉生长的机制尚不清楚。我们的工作旨在评估抗坏血酸补充对帕库鱼（Piaractus mesopotamicus）体外成肌细胞增殖和迁移的影响。为了深入了解抗坏血酸的潜在抗氧化作用，我们还在体外用甲萘醌（menadione）处理成肌细胞，甲萘醌是一种强大的氧化剂。我们的结果表明，补充抗坏血酸的成肌细胞表现出增殖和迁移增加，并能抵抗甲萘醌引起的氧化应激。此外，抗坏血酸增加了抗氧化酶超氧化物歧化酶的活性，并上调了与骨骼肌肉成肌发生和蛋白质合成相关的分子标志物 myog 和 mtor 的表达。这项工作揭示了抗坏血酸对帕库鱼成肌发生机制的直接影响，并强调了抗坏血酸在刺激鱼类骨骼肌肉生长方面的潜在用途。
