Lancaster F, Delaney C, Samorajski T
Biology Department, Texas Woman's University, Houston.
Int J Dev Neurosci. 1989;7(6):581-9. doi: 10.1016/0736-5748(89)90017-8.
Pregnant Long-Evans rats were fed a liquid diet containing ethanol (30% of total calories) during days 3-19 of gestation. Controls were given ad libitum access to liquid diet lacking ethanol, or pair-fed isocaloric amounts based on consumption by the animals in the ethanol group. Brain development of female offspring was evaluated by analysis of electron micrographs of caudate-putamen and visual cortex. Numbers of presynaptic terminals and synaptic junctions (synaptic density) per unit area were compared for 14- and 28-day-old offspring of dams from the three treatment groups. Synaptic density of the caudate-putamen and visual cortex was not affected by ethanol at 14 or 28 days. Although exposure to ethanol during a period comparable to the first two trimesters of human development with minimal or no undernutrition did not affect numerical density of synapses in visual cortex or caudate-putamen, synaptogenesis of caudate-putamen was altered in offspring of pair-fed animals.
在妊娠第3至19天，给怀孕的Long-Evans大鼠喂食含乙醇（占总热量的30%）的液体饲料。对照组大鼠可随意摄取不含乙醇的液体饲料，或根据乙醇组动物的摄入量给予等热量的配对饲料。通过对尾状核-壳核和视皮层的电子显微镜照片分析来评估雌性后代的脑发育。比较了三个处理组母鼠14日龄和28日龄后代每单位面积的突触前终末数量和突触连接（突触密度）。在14天或28天时，尾状核-壳核和视皮层的突触密度不受乙醇影响。尽管在与人类发育的前两个孕期相当的时期内接触乙醇，且营养缺乏极少或没有营养缺乏，但这并未影响视皮层或尾状核-壳核中突触的数量密度，但配对喂养动物的后代尾状核-壳核的突触发生发生了改变。
