Shuler J K, Tryfiates G P
Oncology. 1978;35(2):73-5. doi: 10.1159/000225259.
Cytoplasmic tyrosine aminotransferase (L-tyrosine: 2-oxoglutarate aminotransferase, EC2.6.1.5) was partially purified from host liver and Morris hepatoma No. 7777 grown in pyridoxine depleted rats. The animals were sacrificed six hours following the intraperitoneal administration of hydrocortisone hemisuccinate. Enzyme preparations were subsequently resolved by electrophoresis on polyacrylamide gels. Enzyme activity was detected histochemically in situ on the gels. Six and at least three enzymatically active protein peaks were detected in host liver and the hepatoma, respectively, by this method.
从在缺乏吡哆醇的大鼠体内生长的宿主肝脏和7777号莫里斯肝癌中部分纯化出细胞质酪氨酸转氨酶（L-酪氨酸：2-氧代戊二酸转氨酶，EC2.6.1.5）。在腹腔注射半琥珀酸氢化可的松6小时后处死动物。随后通过聚丙烯酰胺凝胶电泳分离酶制剂。在凝胶上原位进行组织化学检测酶活性。用这种方法在宿主肝脏和肝癌中分别检测到6个和至少3个具有酶活性的蛋白峰。
