Hoskins I A, Katz J, Ordorica S A, Young B K
Department of Obstetrics and Gynecology, New York University Medical Center, NY.
Am J Obstet Gynecol. 1989 Dec;161(6 Pt 1):1543-5. doi: 10.1016/0002-9378(89)90922-8.
Accurate and rapid diagnosis of chorioamnionitis poses a major diagnostic dilemma. We previously reported that leukocyte esterase activity in amniotic fluid, as measured by dipstick assay, could be used as an aid in the diagnosis of chorioamnionitis. This study examines the effectiveness of an in vitro spectrophotometric assay of esterase activity in amniotic fluid. We define baseline levels of esterase activity in uninfected amniotic fluid and demonstrate a quantitative increase when infection is present. Fifty-seven amniotic fluid samples obtained at second- and third-trimester amniocenteses were divided into three parts, one for culture and two for a comparison of esterase activities by the dipstick and spectrophotometric methods. In this study, the spectrophotometric assay, because of its higher specificity and sensitivity in the determination of elevated esterase activity, was shown to be more reliable for predicting chorioamnionitis than either the dipstick or culture method.
绒毛膜羊膜炎的准确快速诊断是一个重大的诊断难题。我们之前报道过，通过试纸条检测法测定的羊水白细胞酯酶活性可辅助绒毛膜羊膜炎的诊断。本研究检测了一种用于体外测定羊水酯酶活性的分光光度法的有效性。我们确定了未感染羊水的酯酶活性基线水平，并证明感染时酯酶活性会定量增加。在孕中期和孕晚期羊膜腔穿刺获取的57份羊水样本被分成三份，一份用于培养，两份用于通过试纸条法和分光光度法比较酯酶活性。在本研究中，分光光度法在测定酯酶活性升高方面具有更高的特异性和敏感性，与试纸条法或培养法相比，它在预测绒毛膜羊膜炎方面更可靠。
