de Broucker Virginie, Hulo Sebastien, Cherot-Kornobis Nathalie, Sobaszek Annie, Edme Jean-Louis
a CHU de Lille , Lille , France.
J Toxicol Environ Health A. 2015;78(11):666-70. doi: 10.1080/15287394.2015.1023915.
Several epidemiological studies have shown the impact on respiratory health of pollution of nitrogen dioxide (NO2), particulate matter (PM10), and ozone (O3) as an environmental mixture. However, the influence of individual components of airborne pollutants is less well known. Our study examined the cumulative effects of a single pollutant, NO2, on sensitized rats by measurement of isoprostane release in exhaled breath condensate (EBC). Three groups of six rats were used: (1) controls (only exposed to air), (2) sensitized and challenged by ovalbumin and exposed to air, and (3) sensitized, challenged by ovalbumin, and exposed to NO(2). There was no marked change in 8-isoprostane levels in EBC of sensitized rats, whereas a significant increase of 8-isoprostane was found in rats sensitized and exposed to NO2. Data indicate effect of exposure to NO2 is evident as increased 8-isoprostane levels in EBC, a relevant marker for assessment of pulmonary inflammation or oxidant stress and conventionally found in EBC of asthmatic subjects.
多项流行病学研究表明，作为一种环境混合物，二氧化氮（NO₂）、颗粒物（PM10）和臭氧（O₃）的污染对呼吸系统健康有影响。然而，空气传播污染物单个成分的影响却鲜为人知。我们的研究通过测量呼出气冷凝物（EBC）中异前列腺素的释放，研究了单一污染物NO₂对致敏大鼠的累积影响。使用了三组，每组六只大鼠：（1）对照组（仅暴露于空气中），（2）用卵清蛋白致敏和激发并暴露于空气中，以及（3）用卵清蛋白致敏、激发并暴露于NO₂中。致敏大鼠的EBC中8-异前列腺素水平没有明显变化，而在致敏并暴露于NO₂的大鼠中发现8-异前列腺素显著增加。数据表明，暴露于NO₂的影响很明显，表现为EBC中8-异前列腺素水平升高，这是评估肺部炎症或氧化应激的一个相关标志物，通常在哮喘患者的EBC中发现。
