Kueng W, Silber E, Eppenberger U
Department of Research, University Clinic Medical School, Basel, Switzerland.
Anal Biochem. 1989 Oct;182(1):16-9. doi: 10.1016/0003-2697(89)90710-0.
The method for cell number measurement in monolayer cultures by crystal violet staining published recently by Gillies et al. (R. G. Gillies, N. Didier, M. Denton (1986) Anal. Biochem. 159, 109-113) was modified and significantly improved. The procedure was adapted for use in 96-well plates since the method is inherently very sensitive. Modifications allowed fast and complete solubilization of dye adsorbed by cell nuclei during staining. Since light absorption of the unstained or destained cell layers is negligible, cell number measurements can be performed in the respective wells. Due to these features, multiple assays may be carried out rapidly using standard 96-well plate readers. In addition, it is shown that the sensitivity of the assay can be varied and easily controlled by choosing the appropriate pH during the staining procedure. This increases the flexibility of the method making it useful for determining cell density of a wide range of different cell types.
吉利斯等人（R.G.吉利斯、N.迪迪埃、M.丹顿，《分析生物化学》，1986年，第159卷，第109 - 113页）最近发表的通过结晶紫染色测量单层培养物中细胞数量的方法经过了修改并得到显著改进。该程序适用于96孔板，因为该方法本身非常灵敏。修改后的方法能够快速且完全地溶解染色过程中细胞核吸附的染料。由于未染色或脱色细胞层的光吸收可忽略不计，因此可以在相应的孔中进行细胞数量测量。基于这些特性，可以使用标准的96孔板读数器快速进行多次测定。此外，研究表明，通过在染色过程中选择合适的pH值，可以改变并轻松控制该测定方法的灵敏度。这增加了该方法的灵活性，使其可用于测定多种不同细胞类型的细胞密度。
