Laughton C
Anal Biochem. 1984 Aug 1;140(2):417-23. doi: 10.1016/0003-2697(84)90187-8.
A method has been developed to determine the relative or actual number of attached cells in microtiter plate wells without making direct cell counts. The procedure is based upon staining total cellular protein with Coomassie brilliant blue G-250, followed by measurement of absorbance at 630 nm in a spectrophotometer designed to read each well of a 96-well microtiter plate. No destaining of cells is required. A linear correlation exists (r = 0.970) between cell number and absorbance over a useful range. Intraplate well-to-well variation is acceptable (CV = 0.101). This method was used to measure the proliferative response of human vascular smooth muscle cells to human serum. It should be useful in other assays involving proliferation of attached cultured cells.
已开发出一种无需直接计数细胞就能确定微量滴定板孔中贴壁细胞相对数量或实际数量的方法。该程序基于用考马斯亮蓝G - 250对总细胞蛋白进行染色，然后在设计用于读取96孔微量滴定板各孔的分光光度计中测量630 nm处的吸光度。无需对细胞进行脱色。在有效范围内，细胞数量与吸光度之间存在线性相关性（r = 0.970）。板内孔间差异可接受（CV = 0.101）。该方法用于测量人血管平滑肌细胞对人血清的增殖反应。它在涉及贴壁培养细胞增殖的其他测定中应该会有用。
