基于F(0)F(1)-ATP酶开发新型生物传感器用于检测辐照牛肉中的2-十二烷基环丁酮。

Development of a novel biosensor based on F(0)F(1)-ATPase for the detection of 2-dodecylcyclobutanone in irradiated beef.

作者信息

Zhao Yueliang, Ha Yiming, Yue Jiachang, Wang Feng

机构信息

Institute of Agro-food Science and Technology, Chinese Academy of Agricultural Sciences/Key Opening Laboratory of Agricultural Products Processing and Quality Control, Ministry of Agriculture, Beijing 100193, China.

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

Food Chem. 2015 Dec 1;188:320-4. doi: 10.1016/j.foodchem.2015.04.142. Epub 2015 May 1.

DOI:10.1016/j.foodchem.2015.04.142

PMID:26041198

Abstract

A novel biosensor regulated by the rotator of F0F1-ATPase was developed to analyze 2-dodecylcyclobutanone (2-DCB) to detect γ-ray irradiated beef rapidly. The biosensor was assembled by conjugating 2-DCB monoclonal antibodies with the "rotator" ε-subunit of F0F1-ATPase within chromatophores through an ε-subunit monoclonal antibody-biotin-avidin-biotin linker. The limit of detection (LOD) of 2-DCB was approximately 10(-8) μg/mL. The recovery ratio of 2-DCB from ground beef patties ranged from 75.1% to 116.4%. The intra-assay and inter-assay coefficients of variation were both <15.0%. The proposed method was validated by gas chromatography-mass spectrometry with high correlation. The biosensor was used to detect 2-DCB in ground beef patties with different fat contents (10%, 20%, and 30%) irradiated at 0.5, 1.0, 3.0, 5.0, and 7.0 kGy. The 2-DCB concentration linearly increased with the radiation dose in all the beef samples. 2-DCB concentration increased with fat levels in the three samples.

摘要

开发了一种由F0F1 - ATP酶旋转体调节的新型生物传感器，用于分析2 - 十二烷基环丁酮（2 - DCB），以快速检测γ射线辐照牛肉。该生物传感器是通过ε - 亚基单克隆抗体 - 生物素 - 抗生物素蛋白 - 生物素连接体，将2 - DCB单克隆抗体与色素细胞内F0F1 - ATP酶的“旋转体”ε - 亚基偶联组装而成。2 - DCB的检测限（LOD）约为10^(-8) μg/mL。牛肉饼中2 - DCB的回收率在75.1%至116.4%之间。批内和批间变异系数均<15.0%。所提出的方法通过气相色谱 - 质谱法进行验证，具有高度相关性。该生物传感器用于检测不同脂肪含量（10%、20%和30%）的牛肉饼在0.5、1.0、3.0、5.0和7.0 kGy辐照下的2 - DCB。在所有牛肉样品中，2 - DCB浓度随辐射剂量呈线性增加。在三个样品中，2 - DCB浓度随脂肪含量增加。