Sallam Ahmed, Martsch Regina
Department of Crop Sciences, Georg-August-Univeristät Göttingen, Von-Siebold-Str. 8, 37075, Göttingen, Germany,
Genetica. 2015 Aug;143(4):501-14. doi: 10.1007/s10709-015-9848-z. Epub 2015 Jun 4.
A multi-parent advanced generation inter-cross (MAGIC) derived from 11 founder lines in faba bean was used in this study to identify quantitative trait loci (QTL) for frost tolerance traits using the association mapping method with 156 SNP markers. This MAGIC population consists of a set of 189 genotypes from the Göttingen Winter Bean Population. The association panel was tested in two different experiments, i.e. a frost and a hardening experiment. Six morphological traits, leaf fatty acid composition, relative water content in shoots were scored in this study. The genotypes presented a large genetic variation for all traits that were highly heritable after frost and after hardening. High phenotypic significant correlations were established between traits. The principal coordinates analysis resulted in no clear structure in the current population. Association mapping was performed using a general linear model and mixed linear model with kinship. A False discovery rate of 0.20 (and 0.05) was used to test the significance of marker-trait association. As a result, many putative QTLs for 13 morphological and physiological traits were detected using both models. The results reveal that QTL mapping by association analysis is a powerful method of detecting the alleles associated with frost tolerance in the winter faba bean which can be used in accelerating breeding programs.
本研究使用了由11个蚕豆亲本系衍生而来的多亲本高代杂交群体（MAGIC），通过关联作图法和156个单核苷酸多态性（SNP）标记来鉴定蚕豆抗冻性性状的数量性状位点（QTL）。这个MAGIC群体由来自哥廷根冬蚕豆群体的189个基因型组成。关联群体在两个不同的试验中进行了测试，即霜冻试验和低温锻炼试验。本研究对六个形态性状、叶片脂肪酸组成、地上部相对含水量进行了评分。这些基因型在所有性状上都表现出较大的遗传变异，在霜冻和低温锻炼后具有较高的遗传力。各性状之间建立了高度显著的表型相关性。主坐标分析在当前群体中未发现明显的结构。使用一般线性模型和带亲缘关系的混合线性模型进行关联作图。使用错误发现率为0.20（和0.05）来检验标记-性状关联的显著性。结果，使用这两种模型都检测到了许多与13个形态和生理性状相关的假定QTL。结果表明，通过关联分析进行QTL定位是检测冬蚕豆中与抗冻性相关等位基因的一种有效方法，可用于加速育种计划。
