Carrillo-Perdomo Estefanía, Magnin-Robert Jean-Bernard, Raffiot Blandine, Deulvot Chrystel, Floriot Matthieu, Lejeune-Hénaut Isabelle, Marget Pascal, Burstin Judith, Tayeh Nadim, Aubert Grégoire
Agroécologie, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.
UMR AGAP Institut, Univ. Montpellier, CIRAD, INRAE, Institut Agro, San Giuliano, France.
Front Plant Sci. 2022 Oct 19;13:970865. doi: 10.3389/fpls.2022.970865. eCollection 2022.
Frost is a major abiotic stress of winter type faba beans ( L.) and has adverse effects on crop yield. Climate change, far from reducing the incidence of frost events, is making these phenomena more and more common, severe, and prolonged. Despite the important interaction that the environment has in the tolerance of faba bean to frost, this trait seems to have good levels of heritability. Several QTLs for frost tolerance have already been reported, however, a more robust identification is needed to more precisely identify the genomic regions involved in faba bean tolerance to sub-zero temperatures. Several pea ( L.) and barrel medic ( L.) frost tolerance QTLs appear to be conserved between these two species, furthering the hypothesis that the genetic control of frost tolerance in legume species might be more generally conserved. In this work, the QTL mapping in two faba bean recombinant inbred line (RIL) populations connected by a common winter-type parent has led to the identification of five genomic regions involved in the control of frost tolerance on linkage groups I, III, IV, and V. Among them, a major and robust QTL of great interest for marker-assisted selection was identified on the lower part of the long-arm of LGI. The synteny between the faba bean frost tolerance QTLs and those previously identified in other legume species such as barrel medic, pea or soybean highlighted at least partial conservation of the genetic control of frost tolerance among different faba bean genetic pools and legume species. Four novel RILs showing high and stable levels of tolerance and the ability to recover from freezing temperatures by accumulating frost tolerance QTLs are now available for breeding programs.
霜冻是冬性蚕豆（L.）的主要非生物胁迫，对作物产量有不利影响。气候变化非但没有减少霜冻事件的发生率，反而使这些现象越来越普遍、严重和持久。尽管环境对蚕豆耐霜冻性有重要影响，但该性状似乎具有较高的遗传力水平。已经报道了几个耐霜冻的数量性状位点（QTL），然而，需要更可靠的鉴定来更精确地确定参与蚕豆耐零下温度的基因组区域。豌豆（L.）和扁蓿豆（L.）的几个耐霜冻QTL在这两个物种之间似乎是保守的，这进一步支持了豆科植物耐霜冻遗传控制可能更普遍保守的假设。在这项研究中，通过一个共同的冬性亲本连接的两个蚕豆重组自交系（RIL）群体中的QTL定位，已确定了连锁群I、III、IV和V上五个参与耐霜冻控制的基因组区域。其中，在LGI长臂下部鉴定出一个对标记辅助选择非常重要且可靠的主效QTL。蚕豆耐霜冻QTL与之前在其他豆科物种如扁蓿豆、豌豆或大豆中鉴定出的QTL之间的共线性，突出了不同蚕豆基因库和豆科物种之间耐霜冻遗传控制至少部分保守。现在有四个新的RILs可供育种计划使用，它们表现出高且稳定的耐受性水平，以及通过积累耐霜冻QTL从冷冻温度中恢复的能力。
