Shabani Leila, Sabzalian Mohammad R, Mostafavi pour Sodabeh
Department of Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran.
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Mycorrhiza. 2016 Jan;26(1):67-76. doi: 10.1007/s00572-015-0647-2. Epub 2015 Jun 4.
Mycorrhizal fungi are key microorganisms for enhancing phytoremediation of soils contaminated with heavy metals. In this study, the effects of the arbuscular mycorrhizal fungus (AMF) Funneliformis mosseae (=Glomus mosseae) on physiological and molecular mechanisms involved in the nickel (Ni) tolerance of tall fescue (Festuca arundinacea = Schedonorus arundinaceus) were investigated. Nickel addition had a pronounced negative effect on tall fescue growth and photosynthetic pigment contents, as well as on AMF colonization. Phosphorus content increased markedly in mycorrhizal plants (M) compared to non-inoculated (NM) ones. However, no significant difference was observed in root carbohydrate content between AMF-inoculated and non-inoculated plants. For both M and NM plants, Ni concentrations in shoots and roots increased according to the addition of the metal into soil, but inoculation with F. mosseae led to significantly lower Ni translocation from roots to the aboveground parts compared to non-inoculated plants. ABC transporter and metallothionein transcripts accumulated to considerably higher levels in tall fescue plants colonized by F. mosseae than in the corresponding non-mycorrhizal plants. These results highlight the importance of mycorrhizal colonization in alleviating Ni-induced stress by reducing Ni transport from roots to shoots of tall fescue plants.
菌根真菌是增强重金属污染土壤植物修复的关键微生物。在本研究中，研究了丛枝菌根真菌（AMF）摩西斗管囊霉（= 摩西球囊霉）对高羊茅（= 苇状羊茅）耐镍生理和分子机制的影响。添加镍对高羊茅生长、光合色素含量以及AMF定殖均有显著负面影响。与未接种（NM）的植株相比，菌根植株（M）中的磷含量显著增加。然而，接种AMF和未接种的植株之间根碳水化合物含量未观察到显著差异。对于M和NM植株，地上部和根部的镍浓度均随土壤中金属添加量的增加而增加，但与未接种植株相比，接种摩西斗管囊霉导致从根部向地上部分的镍转运显著降低。ABC转运蛋白和金属硫蛋白转录本在被摩西斗管囊霉定殖的高羊茅植株中积累的水平明显高于相应的非菌根植株。这些结果突出了菌根定殖在减轻镍诱导的胁迫方面的重要性，即通过减少镍从高羊茅植株根部向地上部的转运来实现。
