Nawaz Aniqa, Shahbaz Muhammad, Imran Asma, Marghoob Muhammad U, Imtiaz Muhammad, Mubeen Fathia
Stress Physiology Lab, Department of Botany, University of Agriculture, Faisalabad, Pakistan.
Microbial Physiology Lab, Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Constituent College of Pakistan Institute of Engineering and Applied Sciences, Islamabad, Pakistan.
Front Microbiol. 2020 Oct 2;11:2019. doi: 10.3389/fmicb.2020.02019. eCollection 2020.
Soil salinity has emerged as a major obstacle to meet world food demands. Halo-tolerant plant growth promoting rhizobacteria (PGPR) are potential bioinoculants to enhance crop productivity in saline agriculture. Current work was aimed at studying individual or synergetic impact of salt tolerant PGPR on wheat growth and yield under saline conditions. A pot experiment was conducted on two wheat genotypes (Aas-11; salt tolerant and Galaxy-13; salt sensitive) inoculated with , and alone and in consortium. The salt tolerant variety (Aas-11) exhibited maximum root fresh (665.2%) and dry biomass (865%), free proline (138.12%) and total soluble proteins (155.9%) contents, CAT (41.7%) activity and shoot potassium uptake (81.08%) upon inoculation with , while improved shoot dry weight (70.39%), water (23.49%) and osmotic (29.65%) potential, POD (60.51%) activity, enhanced root potassium (286.36%) and shoot calcium (400%) were manifested by Highest shoot length (14.38%), fresh weight (72.73%), potassium (29.7%) and calcium (400%) acquisition as well as glycinebetaine (270.31%) content were found in plants treated with PGPR consortium. On the other hand, in the salt sensitive variety (Galaxy-13), treated plants showed significantly improved leaf-water relations, glycinebetaine (10.78%) content, shoot potassium (23.07%), root calcium (50%) uptake, and yield parameters, respectively. Plant root length (71.72%) and potassium content (113.39%), root and shoot fresh and dry biomass, turgor potential (231.02%) and free proline (317.2%) content were maximum upon PGPR inoculation in consortium. Overall, Aas-11 (salt tolerant variety) showed significantly better performance than Galaxy-13 (salt sensitive variety). This study recommends and for the salt tolerant (Aas-11) and for the salt sensitive (Galaxy-13) varieties, as potential bioinoculants to augment their growth and yield through modulation of morpho-physiological and biochemical attributes under saline conditions.
土壤盐渍化已成为满足世界粮食需求的主要障碍。耐盐促生根际细菌（PGPR）是提高盐碱地农业作物生产力的潜在生物接种剂。当前的工作旨在研究耐盐PGPR对盐碱条件下小麦生长和产量的单独或协同影响。对两种小麦基因型（耐盐的Aas - 11和盐敏感的Galaxy - 13）进行盆栽试验，分别接种 、 单独接种以及混合接种。接种 后，耐盐品种（Aas - 11）表现出最大的根鲜重（665.2%）、干生物量（865%）、游离脯氨酸（138.12%）和总可溶性蛋白（155.9%）含量、CAT活性（41.7%）以及地上部钾吸收量（81.08%）；接种 后，地上部干重提高（70.39%）、水分（23.49%）和渗透势（29.65%）改善、POD活性（60.51%）增强、根系钾含量（286.36%）和地上部钙含量（400%）增加。接种PGPR混合菌剂的植株地上部长（14.38%）、鲜重（72.73%）、钾（29.7%）和钙（400%）吸收量以及甘氨酸甜菜碱含量（270.31%）最高。另一方面，在盐敏感品种（Galaxy - 13）中，接种 的植株分别表现出显著改善的叶片水分关系、甘氨酸甜菜碱含量（10.78%）、地上部钾含量（23.07%）、根系钙吸收量（50%）以及产量参数。接种PGPR混合菌剂后，植株根长（71.72%）和钾含量（113.39%）、根和地上部的鲜重和干生物量、膨压势（231.02%）和游离脯氨酸含量（317.2%）最高。总体而言，Aas - 11（耐盐品种）的表现明显优于Galaxy - 13（盐敏感品种）。本研究推荐 用于耐盐品种（Aas - 11）， 用于盐敏感品种（Galaxy - 13），作为潜在的生物接种剂，通过调节盐碱条件下的形态生理和生化特性来提高其生长和产量。
