Wilbrink Maarten H, ten Kate Geert A, Sanders Peter, Gerwig Gerrit J, van Leeuwen Sander S, Sallomons Erik, Klarenbeek Bert, Hage Johannes A, van Vuure Carine A, Dijkhuizen Lubbert, Kamerling Johannis P
†Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
‡NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Padualaan 8, NL-3584 CH Utrecht, The Netherlands.
J Agric Food Chem. 2015 Jul 1;63(25):5976-84. doi: 10.1021/acs.jafc.5b01505. Epub 2015 Jun 22.
Decoration of prebiotic galacto-oligosaccharides (GOS) with sialic acid yields mixtures of GOS and sialylated GOS (Sia-GOS), novel products that are expected to have both prebiotic and antiadhesive functionalities. The recombinantly produced trans-sialidase enzyme from Trypanosoma cruzi (TcTS), an enzyme with the ability to transfer (α2-3)-linked sialic acid from sialogalactoglycans to asialogalactoglycans, was employed to catalyze this sialylation. As sialic acid acceptor substrates, Vivinal GOS and derived fractions of specific degree of polymerization were taken. As sialic acid donor substrates, bovine κ-casein-derived glycomacropeptide [>99% N-acetylneuraminic acid (Neu5Ac); <1% N-glycolylneuraminic acid (Neu5Gc)] and bovine blood plasma glycoprotein mixture (45% Neu5Ac; 55% Neu5Gc) were selected, yielding potential food and feed products, respectively. High-pH anion-exchange chromatography, matrix-assisted laser-desorption ionization time-of-flight mass spectrometry, and nuclear magnetic resonance spectroscopy were used for product analysis.
用唾液酸修饰益生元低聚半乳糖（GOS）可产生GOS和唾液酸化低聚半乳糖（Sia-GOS）的混合物，这些新产品有望同时具有益生元和抗黏附功能。来自克氏锥虫的重组转唾液酸酶（TcTS），一种能够将（α2-3）连接的唾液酸从唾液酸乳糖聚糖转移到去唾液酸乳糖聚糖的酶，被用于催化这种唾液酸化反应。作为唾液酸受体底物，采用了Vivinal GOS和特定聚合度的衍生级分。作为唾液酸供体底物，分别选择了牛κ-酪蛋白衍生的糖巨肽[>99% N-乙酰神经氨酸（Neu5Ac）；<1% N-糖基神经氨酸（Neu5Gc）]和牛血浆糖蛋白混合物（45% Neu5Ac；55% Neu5Gc），分别产生潜在的食品和饲料产品。采用高pH阴离子交换色谱、基质辅助激光解吸电离飞行时间质谱和核磁共振光谱进行产物分析。
