补充弯曲乳杆菌HY7601和植物乳杆菌KY1032这两种益生菌菌株对降低甘油三酯的作用：降低非糖尿病和高甘油三酯血症受试者的空腹血浆溶血磷脂酰胆碱水平。
The triglyceride-lowering effect of supplementation with dual probiotic strains, Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032: Reduction of fasting plasma lysophosphatidylcholines in nondiabetic and hypertriglyceridemic subjects.
作者信息
Ahn H Y, Kim M, Ahn Y-T, Sim J-H, Choi I-D, Lee S-H, Lee J H
机构信息
Interdisciplinary Course of Science for Aging, Yonsei University, Seoul, South Korea.
Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei University, Seoul, South Korea.
出版信息
Nutr Metab Cardiovasc Dis. 2015 Aug;25(8):724-33. doi: 10.1016/j.numecd.2015.05.002. Epub 2015 May 14.
BACKGROUND AND AIMS
This study evaluated the triglyceride (TG)-lowering effects of consuming dual probiotic strains of Lactobacillus curvatus (L. curvatus) HY7601 and Lactobacillus plantarum (L. plantarum) KY1032 on the fasting plasma metabolome.
METHODS AND RESULTS
A randomized, double-blind, placebo-controlled study was conducted on 92 participants with hypertriglyceridemia but without diabetes. Over a 12-week testing period, the probiotic group consumed 2 g of powder containing 5 × 10(9) colony-forming units (cfu) of L. curvatus HY7601 and 5 × 10(9) cfu of L. plantarum KY1032 each day, whereas the placebo group consumed the same product without probiotics. Fasting plasma metabolomes were profiled using UPLC-LTQ-Orbitrap MS. After 12 weeks of treatment, the probiotic group displayed a 20% reduction (p = 0.001) in serum TGs and 25% increases (p=0.001) in apolipoprotein A-V (apoA-V). At the 12-week follow-up assessment, the following 11 plasma metabolites were significantly reduced in the probiotic group than the placebo group: palmitoleamide, palmitic amide, oleamide, and lysophosphatidyl choline (lysoPC) containing C14:0, C16:1, C16:0, C17:0, C18:3, C18:2, C18:1, and C20:3. In the probiotic group, changes (▵) in TG were negatively correlated with ▵ apoA-V, which was positively correlated with ▵ FFA. In addition, ▵ FFA was strongly and positively correlated with ▵ lysoPCs in the probiotic group but not the placebo group.
CONCLUSIONS
The triglyceride-lowering effects of probiotic supplementation, partly through elevated apoA-V, in borderline to moderate hypertriglyceridemic subjects showed reductions in plasma metabolites, fatty acid primary amides and lysoPCs (NCT02215694; http://www.clinicaltrials.gov). Clinical trials: NCT02215694; http://www.clinicaltrials.gov.
背景与目的
本研究评估了食用弯曲乳杆菌（L. curvatus）HY7601和植物乳杆菌（L. plantarum）KY1032这两种益生菌菌株对空腹血浆代谢组的降甘油三酯（TG）作用。
方法与结果
对92名患有高甘油三酯血症但无糖尿病的参与者进行了一项随机、双盲、安慰剂对照研究。在为期12周的测试期内，益生菌组每天食用2克含有5×10⁹菌落形成单位（cfu）的弯曲乳杆菌HY7601和5×10⁹ cfu的植物乳杆菌KY1032的粉末，而安慰剂组食用不含益生菌的相同产品。使用超高效液相色谱-线性离子阱-轨道阱质谱（UPLC-LTQ-Orbitrap MS）对空腹血浆代谢组进行分析。治疗12周后，益生菌组血清TG降低了20%（p = 0.001），载脂蛋白A-V（apoA-V）增加了25%（p = 0.001）。在12周的随访评估中，益生菌组有11种血浆代谢物比安慰剂组显著降低，包括棕榈油酸酰胺、棕榈酰胺、油酸酰胺以及含有C14:0、C16:1、C16:0、C17:0、C18:3、C18:2、C18:1和C20:3的溶血磷脂酰胆碱（lysoPC）。在益生菌组中，TG的变化（▵）与▵ apoA-V呈负相关，而▵ apoA-V与▵游离脂肪酸（FFA）呈正相关。此外，在益生菌组中，▵ FFA与▵ lysoPCs呈强正相关，而在安慰剂组中则无此相关性。
结论
在临界至中度高甘油三酯血症受试者中，补充益生菌的降甘油三酯作用部分通过升高apoA-V实现，同时血浆代谢物、脂肪酸一级酰胺和lysoPCs减少（NCT02215694；http://www.clinicaltrials.gov）。临床试验：NCT02215694；http://www.clinicaltrials.gov。
Zotero 插件